@article{fdi:010084240,
  title = {{B}acteriome diversity of blackflies' gut and association with {O}nchocerca volvulus, the causative agent of onchocerciasis in {M}bam {V}alley ({C}enter {R}egion, {C}ameroon)},
  author = {{E}kangouo, {A}. {E}. and {D}jeunga, {H}. {N}. {C}. and {S}empere, {G}. and {K}amgno, {J}. and {N}jiokou, {F}. and {F}ewou, {P}. {M}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{V}ector control using larvicides is the main alternative strategy to address limits of preventive chemotherapy using ivermectin for the control of onchocerciasis. {H}owever, it remains substantially limited by implementation difficulties, ecological concerns and the resistance of vector populations. {T}herefore, efficient and environmentally safe alternative control strategies are still needed. {T}his study explores the composition of the blackfly bacteriome and its variability in the presence of {O}nchocerca volvulus infection, in order to determine their potential as a novel vector control-based approach to fight onchocerciasis. {A}n entomological survey of a collection of samples was performed in the {B}afia health district, a historical endemic focus for onchocerciasis in {C}ameroon. {A} total of 1270 blackflies were dissected and the infection rate was 10.1%, indicative of ongoing transmission of onchocerciasis in the surveyed communities. {S}equencing process of blackflies' gut {DNA} for bacteria screening revealed 14 phyla and 123 genera, highlighting the diversity of gut blackflies bacterial communities. {E}ight bacteria formed the core of blackfly bacteriome and {W}olbachia was the predominant genus with 73.4% of relative abundance of blackflies' gut bacterial communities. {A}cidomonas and {R}oseanomas genera were significantly abundant among infected blackflies (p = 0.01), whereas other genera such as {B}revibacterium and {F}ructobacillus were associated with the absence of infection (p = 0.0009). {D}ifferences in gut bacterial distribution of blackflies according to their infection status by the parasite suggest a causal relationship between the bacteriome composition and the onset of blackflies' infection by {O}. volvulus or vice versa. {B}lackfly native bacteria are then potentially involved in infection by {O}. volvulus, either by facilitating or preventing the parasite infestation of the vector. {T}hese bacteria represent an interesting potential as a biological tool/target for a novel approach of vector control to fight onchocerciasis.},
  keywords = {onchocerciasis ; blackfly ; {O}nchocerca volvulus ; bacteriome ; next generation sequencing ; {CAMEROUN} ; {MBAM} {VALLEE}},
  booktitle = {},
  journal = {{P}athogens},
  volume = {11},
  numero = {1},
  pages = {44 [17 p.]},
  year = {2022},
  DOI = {10.3390/pathogens11010044},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084240},
}