@article{fdi:010084088,
  title = {{M}ycelia from {P}leurotus sp. (oyster mushroom) : a new wave of antimicrobials, anticancer and antioxidant bio-ingredients},
  author = {{M}orris, {H}.{J}. and {B}eltran, {Y}. and {L}laurado, {G}. and {B}atista, {P}.{L}. and {P}erraud {G}aime, {I}sabelle and {G}arcia, {N}. and {M}oukha, {S}. and {B}ermudez, {R}.{C}. and {C}os, {P}. and {H}ernandez, {E}. and {D}iez, {J}.{C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction: {T}here has been an upsurge of interest in mushrooms, such as {P}leurotus species, as an important source of bioactive compounds. {M}ycelia-submerged culture represents a promising approach to search new safe and healthy myco-products with standardized quality in addition to mushroom fruiting bodies. {M}ethods: {T}he study examined the in vitro antimicrobial, antitumor and antioxidant activities of a hot-water extract from {P}leurotus sp. mycelium. {T}he antimicrobial activity was screened through the activation of the microbial autolytic system of four bacteria and four yeast strains. {T}he anti-proliferative effects on {NB}4 human leukemia cells were measured by flow-cytometry analyses. {T}he antioxidant activity was investigated by the scavenging of {DPPH} and {ABTS} radicals, the reducing power and the inhibition of lipid peroxidation. {R}esults: {T}he extract activated the microbial autolytic system of eight strains: 7 autolyzing strains with intensity values ({I}s) ranging from 2.7% in {C}andida sp. to 36.1% in {S}accharomyces cerevisiae. {T}hus, the microbial autolytic system of the strains tested (including {G}ram-positive and gram-negative bacteria as well as yeasts) could be activated in vitro by mycelial extract. {P}leurotus extract reduced the viability of {NB}4 leukemia cells, particularly at the concentration of 200 µg/m{L} to 82% compared to control cells, and induced apoptosis demonstrated by an increase in annexin {V}-{FITC}+ cells (25% at 200 µg/m{L}). {A}t 10 mg/m{L}, the extract showed the most potent scavenging effects for {DPPH} and {ABTS} radicals (96% and 55%, respectively) and the inhibition of lipid peroxidation (52%). {T}he mushroom extract at 5 mg/m{L} manifested reducing power of 1.105. {A}lthough carbohydrates (76.8%, w/w) appear to be the most important bioactive compounds, secondary metabolites, like phenolics, would also con- tribute to the antioxidant, antimicrobial and anti-proliferative activities. {C}onclusion: {T}he hot-water extract obtained from {P}leurotus mycelium, in light of its in vitro antimicrobial, antitumor and antioxidant effects could be considered a good candidate for developing nutraceuticals and for designing innovative myco-therapeutics and phytocosmetics applications.},
  keywords = {},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {P}hytocosmetics and {N}atural {I}ngredients},
  volume = {4},
  numero = {1},
  pages = {3 [9 ]},
  ISSN = {2374-0639},
  year = {2017},
  DOI = {10.15171/ijpni.2017.03},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084088},
}