@article{fdi:010083959,
  title = {{I}mpairment of gut microbial biotin metabolism and host biotin status in severe obesity : effect of biotin and prebiotic supplementation on improved metabolism},
  author = {{B}elda, {E}. and {V}oland, {L}. and {T}remaroli, {V}. and {F}alony, {G}. and {A}driouch, {S}. and {A}ssmann, {K}. {E}. and {P}rifti, {E}di and {A}ron-{W}isnewsky, {J}. and {D}ebedat, {J}. and {L}e {R}oy, {T}. and {N}ielsen, {T}. and {A}mouyal, {C}. and {A}ndre, {S}. and {A}ndreelli, {F}. and {B}luher, {M}. and {C}hakaroun, {R}. and {C}hilloux, {J}. and {C}oelho, {L}. {P}. and {D}ao, {M}. {C}. and {D}as, {P}. and {F}ellahi, {S}. and {F}orslund, {S}. and {G}alleron, {N}. and {H}ansen, {T}. {H}. and {H}olmes, {B}. and {J}i, {B}. {Y}. and {P}edersen, {H}. {K}. and {L}e, {P}. and {L}e {C}hatelier, {E}. and {L}ewinter, {C}. and {M}anneras-{H}olm, {L}. and {M}arquet, {F}. and {M}yridakis, {A}. and {P}elloux, {V}. and {P}ons, {N}. and {Q}uinquis, {B}. and {R}ouault, {C}. and {R}oume, {H}. and {S}alem, {J}. {E}. and {S}okolovska, {N}. and {S}ondertoft, {N}. {B}. and {T}ouch, {S}. and {V}ieira-{S}ilva, {S}. and {G}alan, {P}. and {H}olst, {J}. and {G}otze, {J}. {P}. and {K}ober, {L}. and {V}estergaard, {H}. and {H}ansen, {T}. and {H}ercberg, {S}. and {O}ppert, {J}. {M}. and {N}ielsen, {J}. and {L}etunic, {I}. and {D}umas, {M}. {E}. and {S}tumvoll, {M}. and {P}edersen, {O}. {B}. and {B}ork, {P}. and {E}hrlich, {S}. {D}. and {Z}ucker, {J}ean-{D}aniel and {B}ackhed, {F}. and {R}aes, {J}. and {C}lement, {K}. and {M}eta{C}ardis {C}onsortium},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjectives {G}ut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. {W}e examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the {M}eta{C}ardis cohort. {W}e focused on the role of {B} vitamins and {B}7/{B}8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. {D}esign {W}e performed metagenomic analyses in 1545 subjects from the {M}eta{C}ardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. {R}esults {S}evere obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. {W}e found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. {I}n mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. {B}ariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. {F}inally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and {B} vitamins, while limiting weight gain and glycaemic deterioration. {C}onclusion {S}trategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.},
  keywords = {},
  booktitle = {},
  journal = {{G}ut},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[18 p.]},
  ISSN = {0017-5749},
  year = {2022},
  DOI = {10.1136/gutjnl-2021-325753},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083959},
}