The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells Kucko A. auteur aut IRD Alché J. D. auteur aut IRD Tranbarger Timothy auteur aut IRD Wilmowicz E. auteur aut IRD text journalArticle eng text/pdf born digital access Yellow lupine is an economically important crop. This species has been used as a great model for abscission processes for several years due to extreme flower abortion, which takes place in the abscission zone (AZ). AZ activation involves modifications of cell walls, membranes, and cellular structure. In this paper, we applied physiological, molecular, biochemical, and instrumental methods to explore lipid-associated changes and the possible involvement of lipid-derived phytohormones - jasmonates (JAs) -in flower AZ activation. Our comprehensive analyses revealed that natural abscission is accompanied by the upregulation of peroxidase, which reflects a disruption of redox balance and/or lipids peroxidation in AZ cell membranes. Redox imbalance was confirmed by appearance of malondialdehyde. Lipid-related processes involved the specific localization and increased level and activity of lipase and LOX, enzymes associated with cell membrane rupture, and JA biosynthesis. Lipid-hydrolyzing phospholipase D, implicated previously in abscission, is also found in naturally active AZs. Observed changes are accompanied by the accumulation of jasmonates, both free jasmonic acid and its methyl ester. The JA derivative exhibited higher biological activity than the nonconjugated form. Overall, our study shed new light on the lipid and phytohormonal regulation of AZ functioning supporting a role of JAs during abscission-associated events. specialized abscission zone flower abscission jasmonates lipase lipoxygenase methyl jasmonate 076 020 Plant Science 316 111173 [11 p.] 2022 0168-9452 https://www.documentation.ird.fr/hor/fdi:010083923 10.1016/j.plantsci.2021.111173 0168-9452 [F B010083923] https://www.documentation.ird.fr/hor/fdi:010083923 https://www.documentation.ird.fr/intranet/publi/2022-02/010083923.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2022-02-04 2025-02-24 fdi:010083923 fre