@article{fdi:010083909,
  title = {{H}igh resolution proteomics of {A}edes aegypti salivary glands infected with either dengue, {Z}ika or chikungunya viruses identify new virus specific and broad antiviral factors},
  author = {{C}howdhury, {A}. and {M}odahl, {C}. {M}. and {M}iss{\'e}, {D}oroth{\'e}e and {K}ini, {R}. {M}. and {P}ompon, {J}ulien},
  editor = {},
  language = {{ENG}},
  abstract = {{A}rboviruses such as dengue ({DENV}), {Z}ika ({ZIKV}) and chikungunya ({CHIKV}) viruses infect close to half a billion people per year, and are primarily transmitted through {A}edes aegypti bites. {I}nfection-induced changes in mosquito salivary glands ({SG}) influence transmission by inducing antiviral immunity, which restricts virus replication in the vector, and by altering saliva composition, which influences skin infection. {H}ere, we profiled {SG} proteome responses to {DENV} serotype 2 ({DENV}2), {ZIKV} and {CHIKV} infections by using high-resolution isobaric-tagged quantitative proteomics. {W}e identified 218 proteins with putative functions in immunity, blood-feeding or related to the cellular machinery. {W}e observed that 58, 27 and 29 proteins were regulated by {DENV}2, {ZIKV} and {CHIKV} infections, respectively. {W}hile the regulation patterns were mostly virus-specific, we separately depleted four uncharacterized proteins that were upregulated by all three viral infections to determine their effects on these viral infections. {O}ur study suggests that gamma-interferon responsive lysosomal thiol-like ({GILT}-like) has an anti-{ZIKV} effect, adenosine deaminase ({ADA}) has an anti-{CHIKV} effect, salivary gland surface protein 1 ({SGS}1) has a pro-{ZIKV} effect and salivary gland broad-spectrum antiviral protein ({SGBAP}) has an antiviral effect against all three viruses. {T}he comprehensive description of {SG} responses to three global pathogenic viruses and the identification of new restriction factors improves our understanding of the molecular mechanisms influencing transmission.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {11},
  numero = {1},
  pages = {23696 [12 ]},
  ISSN = {2045-2322},
  year = {2021},
  DOI = {10.1038/s41598-021-03211-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083909},
}