@article{fdi:010083867,
  title = {{E}ndonuclease-based genotyping of the {RBM} as a method to track the emergence or evolution of {SARS}-{C}o{V}-2 variants},
  author = {{L}opez, {E}. and {B}arthelemy, {M}. and {B}aronti, {C}{\'e}cile and {M}asse, {S}. and {F}alchi, {A}. and {D}urbesson, {F}. and {V}incentelli, {R}. and {L}amballerie, {X}. de and {C}harrel, {R}. and {C}outard, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ince the beginning of the {COVID}-19 pandemics, variants have emerged. {S}ome of them display increased transmissibility and/or resistance to immune response. {M}ost of the mutations involved in the functional adaptation are found in the receptor-binding motif ({RBM}), dose to the interface with the receptor {ACE}2. {W}e thus developed a fast molecular assay to detect mutations in the {RBM} coding sequence. {A}fter amplification, the amplicon is heat-denatured and hybridized with an amplicon of reference. {T}he presence of a mutation can be detected using a mismatch-specific endonuclease and the cleavage pattern is analyzed by capillary electrophoresis. {T}he method was validated on {RNA} of severe acute respiratory syndrome coronavirus 2 ({SARS}-{C}o{V}-2) variants produced in vitro before being implemented for clinical samples. {T}he assay showed 97.8% sensitivity and 97.8% specificity. {T}he procedure can be set up for high-throughput identification of the presence of mutations and serve as a first-line screening to select the samples for full genome sequencing.},
  keywords = {},
  booktitle = {},
  journal = {{I}science},
  volume = {24},
  numero = {11},
  pages = {103329 [11 p.]},
  year = {2021},
  DOI = {10.1016/j.isci.2021.103329},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083867},
}