%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Reshetnyak, G.
%A Jacobs, J. M.
%A Auguy, Florence
%A Sciallano, C.
%A Claude, L.
%A Medina, C.
%A Perez-Quintero, A. L.
%A Comte, Aurore
%A Thomas, E.
%A Bogdanove, A.
%A Koebnik, Ralf
%A Szurek, Boris
%A Dievart, A.
%A Brugidou, Christophe
%A Lacombe, Séverine
%A Cunnac, Sébastien
%T An atypical class of non-coding small RNAs is produced in rice leaves upon bacterial infection
%D 2021
%L fdi:010083857
%G ENG
%J Scientific Reports - Nature
%@ 2045-2322
%M ISI:000731322900050
%N 1
%P 24141 [20 ]
%R 10.1038/s41598-021-03391-9
%U https://www.documentation.ird.fr/hor/fdi:010083857
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-01/010083857.pdf
%V 11
%W Horizon (IRD)
%X Non-coding small RNAs (sRNA) act as mediators of gene silencing and regulate plant growth, development and stress responses. Early insights into plant sRNAs established a role in antiviral defense and they are now extensively studied across plant-microbe interactions. Here, sRNA sequencing discovered a class of sRNA in rice (Oryza sativa) specifically associated with foliar diseases caused by Xanthomonas oryzae bacteria. Xanthomonas-induced small RNAs (xisRNAs) loci were distinctively upregulated in response to diverse virulent strains at an early stage of infection producing a single duplex of 20-22 nt sRNAs. xisRNAs production was dependent on the Type III secretion system, a major bacterial virulence factor for host colonization. xisRNA loci overlap with annotated transcripts sequences, with about half of them encoding protein kinase domain proteins. A number of the corresponding rice cis-genes have documented functions in immune signaling and xisRNA loci predominantly coincide with the coding sequence of a conserved kinase motif. xisRNAs exhibit features of small interfering RNAs and their biosynthesis depend on canonical components OsDCL1 and OsHEN1. xisRNA induction possibly mediates post-transcriptional gene silencing but they do not broadly suppress cis-genes expression on the basis of mRNA-seq data. Overall, our results identify a group of unusual sRNAs with a potential role in plant-microbe interactions.
%$ 076