%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cerapio, J. P.
%A Perrier, M.
%A Pont, F.
%A Tosolini, M.
%A Laurent, C.
%A Bertani, Stéphane
%A Fournie, J. J.
%T Single-cell RNAseq profiling of human gamma delta T lymphocytes in virus-related cancers and COVID-19 disease
%D 2021
%L fdi:010083797
%G ENG
%J Viruses
%K human ; gammadelta ; lymphocyte ; tumor ; COVID-19 ; transcriptome ; single cell ; differentiation ; trajectory
%M ISI:000726850600001
%N 11
%P 2212 [14 ]
%R 10.3390/v13112212
%U https://www.documentation.ird.fr/hor/fdi:010083797
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-01/010083797.pdf
%V 13
%W Horizon (IRD)
%X The detailed characterization of human gamma delta T lymphocyte differentiation at the single-cell transcriptomic (scRNAseq) level in tumors and patients with coronavirus disease 2019 (COVID-19) requires both a reference differentiation trajectory of gamma delta T cells and a robust mapping method for additional gamma delta T lymphocytes. Here, we incepted such a method to characterize thousands of gamma delta T lymphocytes from (n = 95) patients with cancer or adult and pediatric COVID-19 disease. We found that cancer patients with human papillomavirus-positive head and neck squamous cell carcinoma and Epstein-Barr virus-positive Hodgkin's lymphoma have gamma delta tumor-infiltrating T lymphocytes that are more prone to recirculate from the tumor and avoid exhaustion. In COVID-19, both TCRV gamma 9 and TCRV gamma non9 subsets of gamma delta T lymphocytes relocalize from peripheral blood mononuclear cells (PBMC) to the infected lung tissue, where their advanced differentiation, tissue residency, and exhaustion reflect T cell activation. Although severe COVID-19 disease increases both recruitment and exhaustion of gamma delta T lymphocytes in infected lung lesions but not blood, the anti-IL6R therapy with Tocilizumab promotes gamma delta T lymphocyte differentiation in patients with COVID-19. PBMC from pediatric patients with acute COVID-19 disease display similar gamma delta T cell lymphopenia to that seen in adult patients. However, blood gamma delta T cells from children with the COVID-19-related multisystem inflammatory syndrome are not lymphodepleted, but they are differentiated as in healthy PBMC. These findings suggest that some virus-induced memory gamma delta T lymphocytes durably persist in the blood of adults and could subsequently infiltrate and recirculate in tumors.
%$ 050 ; 052 ; 020