@article{fdi:010083783,
  title = {{T}he {T}ype {III} effectome of the symbiotic {B}radyrhizobium vignae strain ors3257},
  author = {{B}usset, {N}icolas and {G}ully, {D}jamel and {T}eulet, {A}lbin and {F}ardoux, {J}o{\¨e}l and {C}amuel, {A}. and {C}ornu, {D}. and {S}everac, {D}. and {G}iraud, {E}ric and {M}ergaert, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}any {B}radyrhizobium strains are able to establish a {N}od factor-independent symbiosis with the leguminous plant {A}eschynomene indica by the use of a type {III} secretion system ({T}3{SS}). {R}ecently, an important advance in the understanding of the molecular factors supporting this symbiosis has been achieved by the in silico identification and functional characterization of 27 putative {T}3{SS} effectors ({T}3{E}s) of {B}radyrhizobium vignae {ORS}3257. {I}n the present study, we experimentally extend this catalog of {T}3{E}s by using a multi-omics approach. {T}ranscriptome analysis under non-inducing and inducing conditions in the {ORS}3257 wild-type strain and the tts{I} mutant revealed that the expression of 18 out of the 27 putative effectors previously identified, is under the control of {T}ts{I}, the global transcriptional regulator of {T}3{SS} and {T}3{E}s. {Q}uantitative shotgun proteome analysis of culture supernatant in the wild type and {T}3{SS} mutant strains confirmed that 15 of the previously determined candidate {T}3{E}s are secreted by the {T}3{SS}. {M}oreover, the combined approaches identified nine additional putative {T}3{E}s and one of them was experimentally validated as a novel effector. {O}ur study underscores the power of combined proteome and transcriptome analyses to complement in silico predictions and produce nearly complete effector catalogs. {T}he establishment of the {ORS}3257 effectome will form the basis for a full appraisal of the symbiotic properties of this strain during its interaction with various host legumes via different processes.},
  keywords = {{B}radyrhizobium ; type {III} secretion system ; effector ; nodulation ; legume ; symbiosis ; nod factor ; proteome ; transcriptome},
  booktitle = {},
  journal = {{B}iomolecules},
  volume = {11},
  numero = {11},
  pages = {1592 [19 p.]},
  year = {2021},
  DOI = {10.3390/biom11111592},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083783},
}