In-house HIV-1 RNA real-time RT-PCR assays : principle, available tests and usefulness in developing countries

In-house HIV-1 RNA real-time RT-PCR assays : principle, available tests and usefulness in developing countries Rouet F. auteur aut IRD Ménan H. auteur aut IRD Viljoen J. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Mandaliya K. auteur aut IRD Valéa D. auteur aut IRD Lien T. X. auteur aut IRD Danaviah S. auteur aut IRD Rousset D. auteur aut IRD Ganon A. auteur aut IRD Nerrienet E. auteur aut IRD text journalArticle eng text/pdf born digital access The principle of currently available licensed HIV-1 RNA assays is based on real-time technologies that continuously monitor the fluorescence emitted by the amplification products. Besides these assays, in-house quantitative (q) real-time reverse transcription (RT)-PCR (RT-qPCR) tests have been developed and evaluated particularly in developing countries, for two main reasons. First, affordable and generalized access to HIV-1 RNA viral load is urgently needed in the context of expected universal access to prevention and antiretroviral treatment programs in these settings. Second, since many non-B subtypes, circulating recombinant forms and unique recombinant forms circulate in these areas, in-house HIV-1 RNA RT-qPCR assays are ideal academic tools to thoroughly evaluate the impact of HIV-1 genetic diversity on the accuracy of HIV-1 RNA quantification, as compared with licensed techniques. To date, at least 15 distinct in-house assays have been designed. They differ by their chemistry and the HIV-1 target sequence (located in gag, Pol-IN or LTR gene). Analytical performances of the tests that have been extensively evaluated appear at least as good as (or even better than) those of approved assays, with regard to HIV-1 strain diversity. Their clinical usefulness has been clearly demonstrated for early diagnosis of pediatric HIV-1 infection and monitoring of highly active antiretroviral therapy efficacy. The LTR-based HIV-1 RNA RT-qPCR assay has been evaluated by several groups under the auspices of the Agence Nationale de Recherches sur le SIDA et les hepatites virales B et C. It exists now as a complete standardized commercial test. specialized Developing country Genetic diversity Hiv-1 Pcr Quantitative Real-time Reverse transcription Rna Viral load 052 Expert Review of Molecular Diagnostics 8 5 635-650 2008 1473-7159 https://www.documentation.ird.fr/hor/fdi:010083650 10.1586/14737159.8.5.635 1473-7159 [F B010083650] https://www.documentation.ird.fr/hor/fdi:010083650 https://www.documentation.ird.fr/intranet/publi/2021-11/010083650.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-12-05 2021-11-29 fdi:010083650 fre