%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Rouet, F.
%A Ménan, H.
%A Viljoen, J.
%A Ngo-Giang-Huong, Nicole
%A Mandaliya, K.
%A Valéa, D.
%A Lien, T. X.
%A Danaviah, S.
%A Rousset, D.
%A Ganon, A.
%A Nerrienet, E.
%T In-house HIV-1 RNA real-time RT-PCR assays : principle, available tests and usefulness in developing countries
%D 2008
%L fdi:010083650
%G ENG
%J Expert Review of Molecular Diagnostics
%@ 1473-7159
%K Developing country ; Genetic diversity ; Hiv-1 ; Pcr ; Quantitative ; Real-time ; Reverse transcription ; Rna ; Viral load
%M ISI:000259749900010
%N 5
%P 635-650
%R 10.1586/14737159.8.5.635
%U https://www.documentation.ird.fr/hor/fdi:010083650
%> https://www.documentation.ird.fr/intranet/publi/2021-11/010083650.pdf
%V 8
%W Horizon (IRD)
%X The principle of currently available licensed HIV-1 RNA assays is based on real-time technologies that continuously monitor the fluorescence emitted by the amplification products. Besides these assays, in-house quantitative (q) real-time reverse transcription (RT)-PCR (RT-qPCR) tests have been developed and evaluated particularly in developing countries, for two main reasons. First, affordable and generalized access to HIV-1 RNA viral load is urgently needed in the context of expected universal access to prevention and antiretroviral treatment programs in these settings. Second, since many non-B subtypes, circulating recombinant forms and unique recombinant forms circulate in these areas, in-house HIV-1 RNA RT-qPCR assays are ideal academic tools to thoroughly evaluate the impact of HIV-1 genetic diversity on the accuracy of HIV-1 RNA quantification, as compared with licensed techniques. To date, at least 15 distinct in-house assays have been designed. They differ by their chemistry and the HIV-1 target sequence (located in gag, Pol-IN or LTR gene). Analytical performances of the tests that have been extensively evaluated appear at least as good as (or even better than) those of approved assays, with regard to HIV-1 strain diversity. Their clinical usefulness has been clearly demonstrated for early diagnosis of pediatric HIV-1 infection and monitoring of highly active antiretroviral therapy efficacy. The LTR-based HIV-1 RNA RT-qPCR assay has been evaluated by several groups under the auspices of the Agence Nationale de Recherches sur le SIDA et les hepatites virales B et C. It exists now as a complete standardized commercial test.
%$ 052