Duminil Jérôme auteur aut IRD Besnard G. auteur aut IRD text bookSection eng text/pdf born digital access Size, structure, and sequence content lability of plant mitochondrial genome (mtDNA) across species has sharply limited its use in taxonomic studies. Historically, mtDNA variation has been first investigated with RFLPs, while the development of universal primers then allowed studying sequence polymorphisms within short genomic regions (<3 kb). The recent advent of NGS technologies now offers new opportunities by greatly facilitating the assembly of longer mtDNA regions, and even full mitogenomes. Phylogenetic works aiming at comparing signals from different genomic compartments (i.e., nucleus, chloroplast, and mitochondria) have been developed on a few plant lineages, and have been shown especially relevant in groups with contrasted inheritance of organelle genomes. This chapter first reviews the main characteristics of mtDNA and the application offered in taxonomic studies. It then presents tips for best sequencing protocol based on NGS data to be routinely used in mtDNA-based phylogenetic studies. specialized 076AMEPLA04 076BOTA01 020BIOCHI Besse P. ed. edt IRD 2222 107-118 New York Humana Press 2021 2222 https://www.documentation.ird.fr/hor/fdi:010083569 10.1007/978-1-0716-0997-2_6 978-1-0716-0996-5 [F B010083569] https://www.documentation.ird.fr/hor/fdi:010083569 https://www.documentation.ird.fr/intranet/publi/2021-11/010083569.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2021-10-26 2025-02-24 fdi:010083569 fre