@incollection{fdi:010083569, title = {{U}tility of the mitochondrial genome in plant taxonomic studies}, author = {{D}uminil, {J}{\'e}r{\^o}me and {B}esnard, {G}.}, editor = {}, language = {{ENG}}, abstract = {{S}ize, structure, and sequence content lability of plant mitochondrial genome (mt{DNA}) across species has sharply limited its use in taxonomic studies. {H}istorically, mt{DNA} variation has been first investigated with {RFLP}s, while the development of universal primers then allowed studying sequence polymorphisms within short genomic regions (<3 kb). {T}he recent advent of {NGS} technologies now offers new opportunities by greatly facilitating the assembly of longer mt{DNA} regions, and even full mitogenomes. {P}hylogenetic works aiming at comparing signals from different genomic compartments (i.e., nucleus, chloroplast, and mitochondria) have been developed on a few plant lineages, and have been shown especially relevant in groups with contrasted inheritance of organelle genomes. {T}his chapter first reviews the main characteristics of mt{DNA} and the application offered in taxonomic studies. {I}t then presents tips for best sequencing protocol based on {NGS} data to be routinely used in mt{DNA}-based phylogenetic studies.}, keywords = {}, booktitle = {{M}olecular plant taxonomy : methods and protocols}, numero = {2222}, pages = {107--118}, address = {{N}ew {Y}ork}, publisher = {{H}umana {P}ress}, series = {{M}ethods in {M}olecular {B}iology}, year = {2021}, DOI = {10.1007/978-1-0716-0997-2_6}, ISBN = {978-1-0716-0996-5}, URL = {https://www.documentation.ird.fr/hor/fdi:010083569}, }