@incollection{fdi:010083448, title = {{I}dentification of extrachromosomal circular forms of active transposable elements using mobilome-seq}, author = {{L}anciano, {S}. and {Z}hang, {P}anpan and {L}lauro, {C}. and {M}irouze, {M}arie}, editor = {}, language = {{ENG}}, abstract = {{A}ctive transposable elements ({TE}s) generate insertion polymorphisms that can be detected through genome resequencing strategies. {H}owever, these techniques may have limitations for organisms with large genomes or for somatic insertions. {H}ere, we present a method that takes advantage of the extrachromosomal circular {DNA} (ecc{DNA}) forms of actively transposing {TE}s in order to detect and characterize active {TE}s in any plant or animal tissue. {M}obilome-seq consists in selectively amplifying and sequencing ecc{DNA}s. {I}t relies on linear digestion of genomic {DNA} followed by rolling circle amplification of circular {DNA}. {B}oth active {DNA} transposons and retrotransposons can be identified using this technique.}, keywords = {}, booktitle = {{P}lant transposable elements : methods and protocols}, numero = {2250}, pages = {87--93}, address = {{N}ew {Y}ork}, publisher = {{H}umana {P}ress - {S}pringer}, series = {{M}ethods in {M}olecular {B}iology}, year = {2021}, DOI = {10.1007/978-1-0716-1134-0_7}, ISBN = {978-1-0716-1133-3}, URL = {https://www.documentation.ird.fr/hor/fdi:010083448}, }