@article{fdi:010083358,
  title = {{H}ighly variable and non-complex diazotroph communities in corals from ambient and high {CO}2 environments},
  author = {{G}eissler, {L}. and {M}eunier, {V}. and {R}adecker, {N}. and {P}erna, {G}. and {R}odolfo-{M}etalpa, {R}iccardo and {H}oulbr{\`e}que, {F}anny and {V}oolstra, {C}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ecological success of corals depends on their association with microalgae and a diverse bacterial assemblage. {O}cean acidification ({OA}), among other stressors, threatens to impair host-microbial metabolic interactions that underlie coral holobiont functioning. {V}olcanic {CO}2 seeps offer a unique opportunity to study the effects of {OA} in natural reef settings and provide insight into the long-term adaptations under a low p{H} environment. {H}ere we compared nitrogen-fixing bacteria (diazotrophs) associated with four coral species ({P}ocillopora damicornis, {G}alaxea fascicularis, {A}cropora secale, and {P}orites rus) collected from {CO}2 seeps at {T}utum {B}ay ({P}apua {N}ew {G}uinea) with those from a nearby ambient {CO}2 site using nif{H} amplicon sequencing to characterize the effects of seawater p{H} on bacterial communities and nitrogen cycling. {D}iazotroph communities were of generally low diversity across all coral species and for both sampling sites. {O}ut of a total of 25 identified diazotroph taxa, 14 were associated with {P}. damicornis, of which 9 were shared across coral species. {N}one of the diazotroph taxa, however, were consistently found across all coral species or across all samples within a species pointing to a high degree of diazotroph community variability. {R}ather, the majority of sampled colonies were dominated by one or two diazotroph taxa of high relative abundance. {P}ocillopora damicornis and {G}alaxea fascicularis that were sampled in both environments showed contrasting community assemblages between sites. {I}n {P}. damicornis, {G}ammaproteobacteria and {C}yanobacteria were prevalent under ambient p{CO}(2), while a single member of the family {R}hodobacteraceae was present at high relative abundance at the high p{CO}(2) site. {C}onversely, in {G}. fascicularis diazotroph communities were indifferent between both sites. {D}iazotroph community changes in response to {OA} seem thus variable within as well as between host species, potentially arguing for haphazard diazotroph community assembly. {T}his warrants further research into the underlying factors structuring diazotroph community assemblages and their functional role in the coral holobiont.},
  keywords = {coral holobiont ; diazotrophy ; nitrogen fixation ; climate change ; ocean acidification ; nif{H} ; next-generation sequencing ; {PAPOUASIE} {NOUVELLE} {GUINEE} ; {TUTUM} {BAIE}},
  booktitle = {},
  journal = {{F}rontiers in {M}arine {S}cience},
  volume = {8},
  numero = {},
  pages = {754682 [14 p.]},
  year = {2021},
  DOI = {10.3389/fmars.2021.754682},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083358},
}