@article{fdi:010083349,
  title = {{RX}am2, a {NLR} from cassava ({M}anihot esculenta) contributes partially to the quantitative resistance to {X}anthomonas phaseoli pv. manihotis},
  author = {{D}iaz-{T}atis, {P}. {A}. and {O}choa, {J}. {C}. and {R}ico, {E}. {M}. and {R}odriguez, {C}. and {M}edina, {A}. and {S}zurek, {B}oris and {C}havarriaga, {P}. and {L}opez, {C}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{K}ey message {T}he overexpression of {RX}am2, a cassava {NLR} (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by d{TALE}s, reduce cassava bacterial blight symptoms. {C}assava ({M}anihot esculenta) is a tropical root crop affected by different pathogens including {X}anthomonas phaseoli pv. manihotis ({X}pm), the causal agent of cassava bacterial blight ({CBB}). {P}revious studies have reported resistance to {CBB} as a quantitative and polygenic character. {T}his study sought to validate the functional role of a {NLR} (nucleotide-binding leucine-rich repeat) associated with a {QTL} to {X}pm strain {CIO}151 called {RX}am2. {T}ransgenic cassava plants overexpressing {RX}am2 were generated and analyzed. {P}lants overexpressing {RX}am2 showed a reduction in bacterial growth to {X}pm strains {CIO}151, 232 and 226. {I}n addition, designer {TALE}s (d{TALE}s) were developed to specifically bind to the {RX}am2 promoter region. {T}he {X}pm strain transformed with d{TALE}s allowed the induction of the {RX}am2 gene expression after inoculation in cassava plants and was associated with a diminution in {CBB} symptoms. {T}hese findings suggest that {RX}am2 contributes to the understanding of the molecular basis of quantitative disease resistance.},
  keywords = {{B}road-spectrum resistance ; {C}assava bacterial blight ; {D}esigner {TALE}s ; {F}unctional validation ; {R}esistance genes},
  booktitle = {},
  journal = {{P}lant {M}olecular {B}iology},
  volume = {109},
  numero = {3},
  pages = {313--324},
  ISSN = {0167-4412},
  year = {2022},
  DOI = {10.1007/s11103-021-01211-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083349},
}