@article{fdi:010083327,
  title = {{O}ptimized {RNA}-silencing strategies for rice ragged stunt virus resistance in rice},
  author = {{L}acombe, {S}{\'e}verine and {B}angratz, {M}artine and {T}a, {H}. {A}. and {N}guyen, {T}. {D}. and {G}antet, {P}ascal and {B}rugidou, {C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice ragged stunt virus ({RRSV}) is one of the most damaging viruses of the rice culture area in south and far-eastern {A}sia. {T}o date, no genetic resistance has been identified and only expensive and non-environmentally friendly chemical treatments are deployed to fight this important disease. {N}on-chemical approaches based on {RNA}-silencing have been developed as resistance strategies against viruses. {H}ere, we optimized classical mi{RNA} and si{RNA}-based strategies to obtain efficient and durable resistance to {RRSV}. mi{RNA}-based strategies are involved in producing artificial mi{RNA} (ami{R}) targeting viral genomes in plants. {C}lassically, only one ami{R} is produced from a single construct. {W}e demonstrated for the first time that two ami{R}s targeting conserved regions of {RRSV} genomes could be transgenically produced in {N}icotiana benthamiana and in rice for a single precursor. {T}ransgenic rice plants producing either one or two ami{R} were produced. {D}espite efficient ami{R} accumulations, mi{RNA}-based strategies with single or double ami{R}s failed to achieve efficient {RRSV} resistance in transformed rice plants. {T}his suggests that this strategy may not be adapted to {RRSV}, which could rapidly evolve to counteract them. {A}nother {RNA}-silencing-based method for viral resistance concerns producing several viral si{RNA}s targeting a viral fragment. {T}hese viral si{RNA}s are produced from an inverted repeat construct carrying the targeted viral fragment. {H}ere, we optimized the inverted repeat construct using a chimeric fragment carrying conserved sequences of three different {RRSV} genes instead of one. {O}f the three selected homozygous transgenic plants, one failed to accumulate the expected si{RNA}. {T}he two other ones accumulated si{RNA}s from either one or three fragments. {A} strong reduction of {RRSV} symptoms was observed only in transgenic plants expressing si{RNA}s. {W}e consequently demonstrated, for the first time, an efficient and environmentally friendly resistance to {RRSV} in rice based on the si{RNA}-mediated strategy.</p>},
  keywords = {{RNA}-silencing ; virus resistance ; {RRSV} ; rice},
  booktitle = {},
  journal = {{P}lants},
  volume = {10},
  numero = {10},
  pages = {2008 [17 ]},
  year = {2021},
  DOI = {10.3390/plants10102008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083327},
}