Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Cells 2547 [22 p.] IL-22 miR-21-5p miR-21-3p keratinocytes psoriasis proliferation 2021 fdi:010083303 ENG Cells ISI:000713178400001 10 10.3390/cells10102547 https://www.documentation.ird.fr/hor/fdi:010083303 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-12/010083303.pdf 10 Horizon (IRD) Psoriasis is a chronic inflammatory skin disease that is mediated by complex crosstalk between immune cells and keratinocytes (KCs). Emerging studies have showed a specific psoriatic microRNAs signature, in which miR-21 is one of the most upregulated and dynamic miRNAs. In this study, we focused our investigations on the passenger miR-21-3p strand, which is poorly studied in skin and in psoriasis pathogenesis. Here, we showed the upregulation of miR-21-3p in an IMQ-induced psoriasiform mouse model. This upregulation was correlated with IL-22 expression and functionality, both in vitro and in vivo, and it occurred via STAT3 and NF-kappa B signaling. We identified a network of differentially expressed genes involved in abnormal proliferation control and immune regulatory genes implicated in the molecular pathogenesis of psoriasis in response to miR-21-3p overexpression in KCs. These results were confirmed by functional assays that validated the proliferative potential of miR-21-3p. All these findings highlight the importance of miR-21-3p, an underestimated miRNA, in psoriasis and provide novel molecular targets for therapeutic purposes.</p> 050 ; 020 UR250