Detection of SARS-CoV-2 in two cats during the second wave of the COVID-19 pandemic in France /Fritz, Matthieu Nesi, N. Denolly, S. Boson, B. Legros, V. Rosolen, S. G. Briend-Marchal, A. Gouilh, M. A. /Leroy, Eric cats mild-respiratory clinical signs NGS One Health SARS-CoV-2 serology Although there are several reports in the literature of SARS-CoV-2 infection in cats, few SARS-CoV-2 sequences from infected cats have been published. In this study, SARS-CoV-2 infection was evaluated in two cats by clinical observation, molecular biology (qPCR and NGS), and serology (microsphere immunoassay and seroneutralization). Following the observation of symptomatic SARS-CoV-2 infection in two cats, infection status was confirmed by RT-qPCR and, in one cat, serological analysis for antibodies against N-protein and S-protein, as well as neutralizing antibodies. Comparative analysis of five SARS-CoV-2 sequence fragments obtained from one of the cats showed that this infection was not with one of the three recently emerged variants of SARS-CoV-2. This study provides additional information on the clinical, molecular, and serological aspects of SARS-CoV-2 infection in cats. 2022 text https://www.documentation.ird.fr/hor/fdi:010083280 fdi:010083280 Fritz Matthieu, Nesi N., Denolly S., Boson B., Legros V., Rosolen S. G., Briend-Marchal A., Gouilh M. A., Leroy Eric. Detection of SARS-CoV-2 in two cats during the second wave of the COVID-19 pandemic in France. 2022, 8 (1), 14-20 EN FRANCE