@article{fdi:010083147,
  title = {{MALDI}-{TOF} mass spectrometry for the identification of freshwater snails from {S}enegal, including intermediate hosts of schistosomes},
  author = {{H}amlili, {F}. {Z}. and {T}hiam, {F}. and {L}aroche, {M}. and {D}iarra, {A}. {Z}. and {D}oucoure, {S}. and {G}aye, {P}. {M}. and {F}all, {C}. {B}. and {F}aye, {B}abacar and {S}okhna, {C}heikh and {S}ow, {D}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}uthor summary {S}chistosomiasis is a parasitic disease, caused by blood flukes of the genus {S}chistosoma. {T}he infective cercariae are released by freshwater snails belonging to three genera: {B}iomphalaria, {B}ulinus and {O}ncomelania. {I}t is one of the most significant neglected infectious diseases in the world. {S}nail identification is tremendously important for monitoring snail populations and schistosomiasis. {I}dentification is currently based on morphological criteria and molecular biology, both of which present several drawbacks. {M}any studies have reported the performance of {MALDI}-{TOF} {MS}, a technology that allows species identification based on their proteins, as a reliable, rapid, and easy-to-use tool in many fields. {T}he aim of our study was to create a snail database and assess the efficiency of {MALDI}-{TOF} {MS} for snail identification. {T}his study shows that {MALDI}-{TOF} {MS} can rapidly identify both frozen and ethanol-stored specimens of different species. {T}hese results support the use of {MALDI}-{TOF} {MS} in the context of epidemiological studies in {S}chistosoma-endemic areas.</p> {F}reshwater snails of the genera {B}iomphalaria, {B}ulinus, and {O}ncomelania are intermediate hosts of schistosomes that cause human schistosomiasis, one of the most significant infectious neglected diseases in the world. {I}dentification of freshwater snails is usually based on morphology and, potentially, {DNA}-based methods, but these have many drawbacks that hamper their use. {MALDI}-{TOF} {MS} has revolutionised clinical microbiology and has emerged in the medical entomology field. {T}his study aims to evaluate {MALDI}-{TOF} {MS} profiling for the identification of both frozen and ethanol-stored snail species using protein extracts from different body parts. {A} total of 530 field specimens belonging to nine species ({B}iomphalaria pfeifferi, {B}ulinus forskalii, {B}ulinus senegalensis, {B}ulinus truncatus, {B}ulinus globosus, {B}ellamya unicolor, {C}leopatra bulimoides, {L}ymnaea natalensis, {M}elanoides tuberculata) and 89 laboratory-reared specimens, including three species ({B}i. pfeifferi, {B}u. forskalii, {B}u. truncatus) were used for this study. {F}or frozen snails, the feet of 127 field and 74 laboratory-reared specimens were used to validate the optimised {MALDI}-{TOF} {MS} protocol. {T}he spectral analysis yielded intra-species reproducibility and inter-species specificity which resulted in the correct identification of all the specimens in blind queries, with log-score values greater than 1.7. {I}n a second step, we demonstrated that {MALDI}-{TOF} {MS} could also be used to identify ethanol-stored snails using proteins extracted from the foot using a specific database including a large number of ethanol preserved specimens. {T}his study shows for the first time that {MALDI}-{TOF} {MS} is a reliable tool for the rapid identification of frozen and ethanol-stored freshwater snails without any malacological expertise.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {15},
  numero = {9},
  pages = {e0009725 [26 ]},
  ISSN = {1935-2735},
  year = {2021},
  DOI = {10.1371/journal.pntd.0009725},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083147},
}