@article{fdi:010082553,
  title = {{L}ipid-free tuna muscle samples are suitable for total mercury analysis},
  author = {{M}edieu, {A}. and {S}ardenne, {F}. and {L}orrain, {A}nne and {B}odin, {N}athalie and {P}azart, {C}. and {L}e {D}elliou, {H}. and {P}oint, {D}avid},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ropical tunas are largely consumed worldwide, providing major nutritional benefits to humans, but also representing the main exposure to methylmercury, a potent neurotoxin that biomagnifies along food webs. {T}he combination of ecological tracers (nitrogen and carbon stable isotopes, delta 15{N} and delta 13{C}) to mercury concentrations in tunas is scarce yet crucial to better characterize the influence of tuna foraging ecology on mercury exposure and bioaccumulation. {G}iven the difficulties to get modern and historical tuna samples, analyses have to be done on available and unique samples. {H}owever, delta 13{C} values are often analysed on lipid-free samples to avoid bias related to lipid content. {W}hile lipid extraction with non-polar solvents is known to have no effect on delta 15{N} values, its impact on mercury concentrations is still unclear. {W}e used white muscle tissues of three tropical tuna species to evaluate the efficiency and repeatability of different lipid extraction protocols commonly used in delta 13{C} and delta 15{N} analysis. {D}ichloromethane was more efficient than cyclohexane in extracting lipids in tuna muscle, while the automated method appeared more efficient but as repeatable as the manual method. {L}ipid extraction with dichloromethane had no effect on mercury concentrations. {T}his may result from i) the affinity of methylmercury to proteins in tuna flesh, ii) the low lipid content in tropical tuna muscle samples, and iii) the non-polar nature of dichloromethane. {O}ur study suggests that lipid-free samples, usually prepared for tropical tuna foraging ecology research, can be used equivalently to bulk samples to document in parallel mercury concentrations at a global scale.},
  keywords = {{M}ethylmercury ; {F}at content ; {D}elipidation ; {Y}ellowfin ; {B}igeye ; {S}kipjack ; {OCEAN} {INDIEN} ; {SEYCHELLES} ; {ZONE} {TROPICALE} ; {VICTORIA}},
  booktitle = {},
  journal = {{M}arine {E}nvironmental {R}esearch},
  volume = {169},
  numero = {},
  pages = {105385 [6 ]},
  ISSN = {0141-1136},
  year = {2021},
  DOI = {10.1016/j.marenvres.2021.105385},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082553},
}