@article{fdi:010082402,
  title = {{I}solation, identification, and gene expression analysis of the main digestive enzymes during ontogeny of the {N}eotropical catfish {P}seudoplatystoma punctifer ({C}astelnau, 1855)},
  author = {{C}astro-{R}uiz, {D}. and {A}ndree, {K}.{B}. and {B}londeau-{B}idet, {E}. and {F}ernández-{M}{\'e}ndez, {C}. and {G}arcía-{D}ávila, {C}. and {G}isbert, {E}. and {D}arias, {M}aria {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ontogeny of the digestive capacities in fish is species-specific and its knowledge is essential for understanding the nutritional requirements of fish larvae. {W}hile the {A}mazon basin contains the world's highest fish biodiversity, the molecular basis of the early digestive physiology has not yet been reported in any {A}mazonian fish species. {I}n order to increase basic knowledge on the molecular ontogeny of the digestive system of a commercially important {A}mazonian fish species, six digestive enzyme genes (?-amylase, phospholipase {A}2, lipoprotein lipase, trypsin, chymotrypsin, and pepsin) were isolated and identified, and their expression analyzed from 3 to 24 days post fertilization (dpf) in {P}seudoplatystoma punctifer, a catfish species with high potential for aquaculture diversification. {T}he present results, together with those previously obtained on the development of the digestive enzyme activity, showed that gene expression and enzymatic activities are synchronized and that the enzymatic machinery of {P}. punctifer was completely prepared for the onset of exogenous feeding (4 dpf, 6 mm total length, {TL}) and had reached its maturity between 10 and 13 dpf (11-14 mm {TL}). {T}his indicated that the transition from the larval to the juvenile mode of digestion had been completed and they were suitable for weaning under culture conditions. {F}urthermore, the gene expression analyses suggest that this species displays an omnivorous behavior with a preference towards carnivory during early development. {T}he present study provides the first comprehensive ontogenetic analysis of the digestive function from a molecular point of view of a species of the genus {P}seudoplatystoma, and contributes to the development of feeding strategies in the context of {S}outh {A}merican aquaculture diversification.},
  keywords = {{PEROU} ; {AMAZONIE} ; {AMAZONE} {BASSIN}},
  booktitle = {},
  journal = {{A}quaculture},
  volume = {543},
  numero = {},
  pages = {737031 [16 ]},
  ISSN = {0044-8486},
  year = {2021},
  DOI = {10.1016/j.aquaculture.2021.737031},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082402},
}