Nguyen T. H. auteur aut IRD To Thi Mai H. auteur aut IRD Moukouanga Daniel auteur aut IRD Lebrun Michel auteur aut IRD Bellafiore Stéphane auteur aut IRD Champion Antony auteur aut IRD text bookSection eng text/pdf born digital access The function of Jasmonate (JA) is well documented in different plant physiological processes as well as in the interactions with their environment. Mutants impaired in JA production and/or signaling are useful materials to study the function of this phytohormone. Genes involved in the JA biosynthesis pathway in ricehave been described, but few mutants deficient in JA production and signaling have been identified.Moreover, these mutants are mostly generated through random mutagenesis approaches, such as irradiation, EMS treatment, or T-DNA insertion, and potentially harbor undesired mutations that could affect other biological processes. The CRISPR/Cas9 system is a precise and efficient genome editing tool that creates DNA modification at specific loci and limit undesired mutations. In this chapter, we describe a procedure to generate new JA-deficient mutant using CRISPR/Cas9 system in rice. The Allene Oxide Cyclase (OsAOC) gene is targeted since it is a single copy gene in the JA biosynthesis pathway in rice. The widely used variety Oryza sativa japonica Kitaake has been chosen due toits short life cycle and its ease of genetic transformation. This protocol describes the selection of the 20-nttarget sequence, construction of the binary vector, and strategy for selecting the T-DNA-free mutant. specialized 076 084 Champion Antony ed. edt IRD Laplaze Laurent ed. edt IRD 199-209 New York Humana Press 2020 https://www.documentation.ird.fr/hor/fdi:010082340 10.1007/978-1-0716-0142-6_15 978-1-0716-0141-9 [F A010082337] https://www.documentation.ird.fr/hor/fdi:010082340 https://www.documentation.ird.fr/intranet/publi/2021-07/010082340.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2021-06-18 2025-02-24 fdi:010082340 fre