@incollection{fdi:010082340,
  title = {{CRISPR}/{C}as9-mediated gene editing of the jasmonate biosynthesis {O}s{AOC} gene in rice},
  author = {{N}guyen, {T}. {H}. and {T}o {T}hi {M}ai, {H}. and {M}oukouanga, {D}aniel and {L}ebrun, {M}ichel and {B}ellafiore, {S}t{\'e}phane and {C}hampion, {A}ntony},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he function of {J}asmonate ({JA}) is well documented in different plant physiological processes as well as in the interactions with their environment. {M}utants impaired in {JA} production and/or signaling are useful materials to study the function of this phytohormone. {G}enes involved in the {JA} biosynthesis pathway in ricehave been described, but few mutants deficient in {JA} production and signaling have been identified.{M}oreover, these mutants are mostly generated through random mutagenesis approaches, such as irradiation, {EMS} treatment, or {T}-{DNA} insertion, and potentially harbor undesired mutations that could affect other biological processes. {T}he {CRISPR}/{C}as9 system is a precise and efficient genome editing tool that creates {DNA} modification at specific loci and limit undesired mutations. {I}n this chapter, we describe a procedure to generate new {JA}-deficient mutant using {CRISPR}/{C}as9 system in rice. {T}he {A}llene {O}xide {C}yclase ({O}s{AOC}) gene is targeted since it is a single copy gene in the {JA} biosynthesis pathway in rice. {T}he widely used variety {O}ryza sativa japonica {K}itaake has been chosen due toits short life cycle and its ease of genetic transformation. {T}his protocol describes the selection of the 20-nttarget sequence, construction of the binary vector, and strategy for selecting the {T}-{DNA}-free mutant.},
  keywords = {},
  booktitle = {{J}asmonate in plant biology : methods and protocols},
  numero = {},
  pages = {199--209},
  address = {{N}ew {Y}ork},
  publisher = {{H}umana {P}ress},
  series = {{M}ethods in {M}olecular {B}iology},
  year = {2020},
  DOI = {10.1007/978-1-0716-0142-6_15},
  ISBN = {978-1-0716-0141-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082340},
}