@article{fdi:010082317,
  title = {{P}henotypic and genetic characterization of {MERS} coronaviruses from {A}frica to understand their zoonotic potential},
  author = {{Z}hou, {Z}. {Q}. and {H}ui, {K}. {P}. {Y}. and {S}o, {R}. {T}. {Y}. and {L}v, {H}. {B}. and {P}erera, {R}. {A}. {P}. {M}. and {C}hu, {D}. {K}. {W}. and {G}elaye, {E}. and {O}yas, {H}. and {N}jagi, {O}. and {A}bayneh, {T}. and {K}uria, {W}. and {W}alelign, {E}. and {W}anglia, {R}. and {E}l {M}asry, {I}. and {V}on {D}obschuetz, {S}. and {K}alpravidh, {W}. and {C}hevalier, {V}. and {M}iguel, {E}ve and {F}assi-{F}ihri, {O}. and {T}rarore, {A}. and {L}iang, {W}. {W}. and {W}ang, {Y}. {Q}. and {N}icholls, {J}. {M}. and {Z}hao, {J}. {C}. and {C}han, {M}. {C}. {W}. and {P}oon, {L}. {L}. {M}. and {M}ok, {C}. {K}. {P}. and {P}eiris, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}oronaviruses are pathogens of pandemic potential. {M}iddle {E}ast respiratory syndrome coronavirus ({MERS}-{C}o{V}) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. {M}ore than 70% of {MERS}-{C}o{V}-infected dromedaries are found in {E}ast, {N}orth, and {W}est {A}frica, but zoonotic {MERS} disease is only reported from the {A}rabian {P}eninsula. {W}e compared viral replication competence of clade {A} and {B} viruses from the {A}rabian {P}eninsula with genetically diverse clade {C} viruses found in {E}ast ({E}gypt, {K}enya, and {E}thiopia), {N}orth ({M}orocco), and {W}est ({N}igeria and {B}urkina {F}aso) {A}frica. {V}iruses from {A}frica had lower replication competence in ex vivo cultures of the human lung and in lungs of experimentally infected human-{DPP}4 (h{DPP}4) knockin mice. {W}e used lentivirus pseudo types expressing {MERS}-{C}o{V} spike from {S}audi {A}rabian clade {A} prototype strain ({EMC}) or {A}frican clade {C}1.1 viruses and demonstrated that clade {C}1.1 spike was associated with reduced virus entry into the respiratory epithelial cell line {C}alu-3. {I}sogenic {EMC} viruses with spike protein from {EMC} or clade {C}1.1 generated by reverse genetics showed that the clade {C}1.1 spike was associated with reduced virus replication competence in {C}alu-3 cells in vitro, in ex vivo human bronchus, and in lungs of h{DPP}4 knockin mice in vivo. {T}hese findings may explain why zoonotic {MERS} disease has not been reported from {A}frica so far, despite exposure to and infection with {MERS}-{C}o{V}.},
  keywords = {{MERS}-{C}o{V} ; coronaviruses ; {A}frica ; phenotype ; characterization ; {AFRIQUE} {DE} {L}'{EST} ; {AFRIQUE} {DU} {NORD} ; {AFRIQUE} {DE} l'{OUEST} ; {EGYPTE} ; {KENYA} ; {ETHIOPIE} ; {MAROC} ; {NIGERIA} ; {BURKINA} {FASO} ; {ARABIQUE} {PENINSULE} ; {ARABIE} {SAOUDITE}},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {118},
  numero = {25},
  pages = {e2103984118 [8 p.]},
  ISSN = {0027-8424},
  year = {2021},
  DOI = {10.1073/pnas.2103984118},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082317},
}