@article{fdi:010082113,
  title = {{L}imitations of {PCR} detection of filarial {DNA} in human stools from subjects non-infected with soil-transmitted helminths},
  author = {{D}oret, {M}. {P}. {M}. and {N}ana-{D}jeunga, {H}. {C}. and {N}zune-{T}oche, {N}. and {P}ion, {S}{\'e}bastien and {C}hesnais, {C}{\'e}dric and {B}oussinesq, {M}ichel and {K}amgno, {J}. and {V}arlet-{M}arie, {E}. and {L}ocatelli, {S}abrina},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. {R}ecently, filarial {DNA} has been detected in fecal samples from non-human primates in {C}entral {A}frica. {T}he aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12{S} r{DNA}, {C}ox1, {ITS}1 and {LL}20-15k{D}a ladder antigen-gene by conventional {PCR} in {DNA} extracted from stool samples of 52 people infected with {M}ansonella perstans and/or {L}oa loa. {O}f these, 10 patients were infected with soil-transmitted helminths ({T}richuris trichiura and/or {A}scaris lumbricoides), and none were positive for {N}ecator americanus. {I}nterestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. {F}uture studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial {DNA} fragments in stool samples.},
  keywords = {{M}ansonella perstans ; {L}oa loa ; {S}tool sample ; {PCR} ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{P}arasite},
  volume = {28},
  numero = {},
  pages = {47 [6 ]},
  ISSN = {1252-607{X}},
  year = {2021},
  DOI = {10.1051/parasite/2021046},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082113},
}