@article{fdi:010082111,
  title = {{A} non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of {P}lasmodium falciparum in individual mosquito vectors},
  author = {{G}uissou, {E}. and {W}aite, {J}. {L}. and {J}ones, {M}. and {B}ell, {A}. {S}. and {S}uh, {E}. and {Y}ameogo, {K}. {B}. and {D}j{\'e}gb{\'e}, {N}. and {D}a, {D}. {F}. and {H}ien, {D}fds and {Y}erbanga, {R}. {S}. and {O}uedraogo, {A}. {G}. and {D}abir{\'e}, {K}. {R}. and {C}ohuet, {A}nna and {T}homas, {M}. {B}. and {L}ef{\`e}vre, {T}hierry},
  editor = {},
  language = {{ENG}},
  abstract = {{D}espite its epidemiological importance, the time {P}lasmodium parasites take to achieve development in the vector mosquito (the extrinsic incubation period, {EIP}) remains poorly characterized. {A} novel non-destructive assay designed to estimate {EIP} in single mosquitoes, and more broadly to study {P}lasmodium-{A}nopheles vectors interactions, is presented. {T}he assay uses small pieces of cotton wool soaked in sugar solution to collect malaria sporozoites from individual mosquitoes during sugar feeding to monitor infection status over time. {T}his technique has been tested across four natural malaria mosquito species of {A}frica and {A}sia, infected with {P}lasmodium falciparum (six field isolates from gametocyte-infected patients in {B}urkina {F}aso and the {NF}54 strain) and across a range of temperatures relevant to malaria transmission in field conditions. {M}onitoring individual infectious mosquitoes was feasible. {T}he estimated median {EIP} of {P}. falciparum at 27 degrees {C} was 11 to 14 days depending on mosquito species and parasite isolate. {L}ong-term individual tracking revealed that sporozoites transfer onto cotton wool can occur at least until day 40 post-infection. {S}hort individual {EIP} were associated with short mosquito lifespan. {C}orrelations between mosquito/parasite traits often reveal trade-offs and constraints and have important implications for understanding the evolution of parasite transmission strategies.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {11},
  numero = {1},
  pages = {9344 [14 ]},
  ISSN = {2045-2322},
  year = {2021},
  DOI = {10.1038/s41598-021-88659-w},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082111},
}