Gondiho Mendes R.A. auteur aut IRD Basso M.F. auteur aut IRD Melo B.P. de auteur aut IRD Pires Ribeiro T. auteur aut IRD Lima R.N. auteur aut IRD Araujo J. F. de auteur aut IRD Grossi-de-Sa M. auteur aut IRD Silva Mattos V. da auteur aut IRD Coiti Togawa R. auteur aut IRD Saliba Albuquerque E.V. auteur aut IRD Lisei de Sa M.E. auteur aut IRD Mattar da Silva M.C. auteur aut IRD Macedo L.L.P. auteur aut IRD Rocha Fragoso D. da auteur aut IRD Fernandez Diana auteur aut IRD Vignols F. auteur aut IRD Grossi-de-Sa M.F. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Meloidogyne incognita is the most frequently reported specie in the root-knot nematode (RKN) responsible for damage to several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and promote plant parasitism. On the other hand, several plant elements are associated with the immune defense system that opposes nematode infection. In this study, the interaction of the Mi-EFF1/Minc17998 effector with the soybean GmHub6 (Glyma.17G099100; TCP14) protein was identified and characterized in vivo and in planta. Data showed that the GmHub6 gene is upregulated by M. incognita infection in a nematode-resistant soybean genotype (PI595099) compared to a susceptible cultivar (BRS133). As a result, the Arabidopsis thaliana AtHub6 mutant (AtHub6KO) line (AT3G47620, an orthologous gene of GmHub6) exhibited normal vegetative development of the plant but was more susceptible to M. incognita. Thus, since the soybean and A. thaliana Hub6 proteins are TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors involved in plant development and morphogenesis modulation, flowering time regulation, and activation of the plant immune system, our data suggest that the interaction of Mi-EFF1/Minc17998 and Hub6 proteins is associated with an increase in plant susceptibility to nematode infection during parasitism. It is suggested that this interaction may prevent the nuclear localization or disturb the activity of GmHub6 as a typical transcription factor modulating the cell cycle of the plant, avoid the activation of the host's defense response, and successfully promote parasitism. Our findings indicate the potential of the Mi-EFF1/Minc17998 effector for the development of biotechnological tools based on the RNA interference approaches and GmHub6 gene overexpression for the RKN control. specialized MONDE 076RAVPLA 114 101630 [11 ] 2021 0885-5765 https://www.documentation.ird.fr/hor/fdi:010081995 10.1016/j.pmpp.2021.101630 0885-5765 [F B010081995] https://www.documentation.ird.fr/hor/fdi:010081995 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-07/010081995.pdf IRD - Base Horizon / Pleins textes 2021-06-16 2025-02-24 fdi:010081995 fre