@article{fdi:010081628,
  title = {{M}icrosatellite variability in the entomopathogenic fungus {P}aecilomyces fumosoroseus : genetic diversity and population structure},
  author = {{G}authier, {N}athalie and {D}alleau {C}louet, {C}. and {F}argues, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he hyphomycete {P}aecilomyces fumosoroseus ({P}fr) is a geographically widespread fungus capable of infecting various insect hosts. {T}he fungus has been used for the biological control of several important insect pests of agriculture. {H}owever knowledge of the fungus' genetic diversity and population structure is required for its sustainable use as a biological control agent. {W}e investigated length and sequence polymorphisms of nine microsatellite loci for 33 {P}fr accessions sampled from various host species and geographical locations, and our results reveal complex mutational processes for these molecular markers. {O}nly {P}fr isolates from {B}emisia tabaci were amplified successfully, indicating the existence of {P}fr genotypes specifically associated with {B}. tabaci. {G}enetic relationships among the 25 {P}fr isolates from {B}. tabaci were inferred from allelic variability data at eight microsatellite loci that were polymorphic and subsequently from sequence data from the flanking regions of three selected loci. {M}aximum parsimony and neighbor joining analyses partitioned {P}fr genetic diversity in two major lineages. {O}ne lineage included genotypes from the {B}-biotype of {B}. tabaci distributed across the {A}mericas and was strongly supported in both analyses. {A}nother lineage was distributed across {A}sia and consisted of four distinct clusters. {A}llele size homoplasy was found at the three microsatellite loci. {W}e obtained better discrimination and resolution of the relationships among isolates with sequence data, although not all isolates could be typed. {T}hus sequencing of microsatellite flanking regions and repeats is a promising approach for the identification of {P}fr isolates that specifically infect certain {B}. tabaci biotypes and phylogeographic studies.},
  keywords = {{B}emisia tabaci host influence ; genetic relationships ; length and sequence polymorphisms ; microsatellites ; mutational patterns},
  booktitle = {},
  journal = {{M}ycologia},
  volume = {99},
  numero = {5},
  pages = {693--704},
  ISSN = {0027-5514},
  year = {2007},
  DOI = {10.3852/mycologia.99.5.693},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081628},
}