@article{fdi:010081268,
  title = {{B}iochar and cow manure on chemical and microbial community in regosol with bean},
  author = {da {S}ilva, {C}. {C}. {G}. and de {M}edeiros, {E}. {V}. and {F}racetto, {G}. {G}. {M}. and {F}racetto, {F}. {J}. {C}. and {M}artins, {A}. {P}. and {L}ima, {J}. {R}. {D}. and {D}uda, {G}. {P}. and da {C}osta, {D}. {P}. and {H}ammecker, {C}laude},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he effects of coffee biochar residue combined with cow manure fertilizer on soil chemical attributes, microbial biomass, and structure and abundance of the soil microbial community are not fully understood. {T}hus, the objective of this study was to evaluate the effect of coffee biochar combined with cow manure on chemical and microbial attributes in soil cultivated with bean ({P}haseolus vulgaris). {W}e hypothesized that biochar from coffee residues drives the bacteria, fungi, and diazotrophic communities in a sandy soil and increases the abundance of these microorganism groups, raising soil basal respiration and microbial biomass. {T}he experiment was carried out in a completely randomized design and was distributed in a factorial scheme (2 x 3 + 2) including two types of biochar (coffee ground-{CG} and coffee husk-{CH}) at three dosages (4, 8, and 16 t ha(-1)), and two control groups ({S} = native soil without treatment (-control) and {E} = fertilization with cow manure at dosage of 15 m(3) ha(-1) (+control)). {T}he experiments were conducted with four repetitions. {A}ll treatments received the same dosage of cow manure applied in sandy soil and bean seeds were grown in pots. {S}oil samples were collected after 45 days to evaluate chemical attributes, total organic carbon ({TOC}), microbial biomass ({C}, {N}, and {P}), soil basal respiration, structure and abundance of total bacterial and fungal community (16{S} r{RNA}, 18{S} r{RNA}) and diazotrophs (nif{H}) in the soil by {DGGE} (denaturing gradient gel electrophoresis) and q{PCR} (real-time quantitative {PCR}), respectively. {T}he results showed that {TOC} increased 9 and 11.5% with highest dosage of {CG} and {CH} biochar, respectively; thus, {CG} at 4 t ha(-1) increased the {C}:{N} ratio in soil (4.7 times). {T}he addition of biochar combined with cow manure increased soil basal respiration in {CG} at 16 t ha(-1). {T}he combined application of {CH} at 16 t ha(-1) and cow manure increased the {P} content (2.37 times) and {K}+ (5.6 times). {T}he highest increase in {C} from microbial biomass was found in {CG} at 16 t ha(-1) and the addition of biochar and cow manure did not affect the {P} content in microbial biomass; nevertheless, biochar increased the nif{H} gene abundance by 8.23% at 16 t ha(-1). {B}iochar addition influenced the structure of soil microbial communities, presenting distinct and well-differentiated communities in the multivariate analysis, according to the dosage and type of biochar. {T}he group formed by different {CG} dosages showed a higher number of 16{S} genes, and greater amounts of {N}mic, nif{H}, soil basal respiration, metabolic quotient, {K}, and {N}a. {T}he major conclusion of this study was that coffee biochar, used in combination with cow manure, significantly improves the chemical and microbiological attributes of sandy soil cultivated with bean.},
  keywords = {{B}iocarbon ; {A}groindustrial by-products ; {S}oil microbial biomass ; 16{S} r{RNA} ; 18{S} r{RNA} ; nif{H} ; {BRESIL} ; {PERNAMBUCO} ; {ZONE} {TROPICALE} ; {ZONE} {SEMIARIDE}},
  booktitle = {},
  journal = {{J}ournal of {S}oil {S}cience and {P}lant {N}utrition},
  volume = {21},
  numero = {2},
  pages = {1552--1564},
  ISSN = {0718-9508},
  year = {2021},
  DOI = {10.1007/s42729-021-00461-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081268},
}