@article{fdi:010081257,
  title = {{I}g{G} acquisition against {P}f{EMP}1 {PF}11_0521 domain cassette {DC}13, {DBL} beta 3_{D}4 domain, and peptides located within these constructs in children with cerebral malaria},
  author = {{B}adaut, {C}. and {V}isitdesotrakul, {P}. and {C}habry, {A}. and {B}igey, {P}. and {T}ornyigah, {B}. and {R}oman, {J}ocelyne and {M}aroufou, {J}. {A}. and {A}moussou, {A}. and {A}yivi, {B}. {S}. and {S}agbo, {G}. and {T}uikue {N}dam, {N}icaise and {O}leinikov, {A}. {V}. and {T}ahar, {R}achida},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {P}lasmodium falciparum erythrocyte-membrane-protein-1 ({PF}3{D}7_1150400/{PF}11_0521) contains both domain cassette {DC}13 and {DBL} beta 3 domain binding to {EPCR} and {ICAM}-1 receptors, respectively. {T}his type of {P}f{EMP}1 proteins with dual binding specificity mediate specific interactions with brain micro-vessels endothelium leading to the development of cerebral malaria ({CM}). {U}sing plasma collected from children at time of hospital admission and after 30 days, we study an acquisition of {I}g{G} response to {PF}3{D}7_1150400/{PF}11_0521 {DC}13 and {DBL} beta 3_{D}4 recombinant constructs, and five peptides located within these constructs, specifically in {DBL} alpha 1.7_{D}2 and {DBL} beta 3_{D}4 domains. {W}e found significant {I}g{G} responses against the entire {DC}13, {PF}11_0521_{DBL} beta 3_{D}4 domain, and peptides. {T}he responses varied against different peptides and depended on the clinical status of children. {T}he response was stronger at day 30, and mostly did not differ between {CM} and uncomplicated malaria ({UM}) groups. {S}pecifically, the {DBL} beta 3 {B}3-34 peptide that contains essential residues involved in the interaction between {PF}11_0521 {DBL} beta 3_{D}4 domain and {ICAM}-1 receptor demonstrated significant increase in reactivity to {I}g{G}1 and {I}g{G}3 antibodies at convalescence. {F}urther, {I}g{G} reactivity in {CM} group at time of admission against functionally active ({ICAM}-1-binding) {PF}11_0521 {DBL} beta 3_{D}4 domain was associated with protection against severe anemia. {T}hese results support development of vaccine based on the {PF}3{D}7_1150400/{PF}11_0521 structures to prevent {CM}.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {11},
  numero = {1},
  pages = {3680 [12 p.]},
  ISSN = {2045-2322},
  year = {2021},
  DOI = {10.1038/s41598-021-82444-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081257},
}