@article{fdi:010081166,
  title = {{A} {PCR}-{RFLP} method for genotyping of inversion 2{R}c in {A}nopheles coluzzii},
  author = {{M}ontanez-{G}onzalez, {R}. and {V}allera, {A}. {C}. and {C}alzetta, {M}. and {P}ichler, {V}. and {L}ove, {R}. {R}. and {G}uelbeogo, {M}. {W}. and {D}abire, {R}. {K}. and {P}ombi, {M}. and {C}ostantini, {C}arlo and {S}imard, {F}r{\'e}d{\'e}ric and della {T}orre, {A}. and {B}esansky, {N}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {G}enotyping of polymorphic chromosomal inversions in malaria vectors such as {A}n. coluzzii {C}oetzee & {W}ilkerson is important, both because they cause cryptic population structure that can mislead vector analysis and control and because they influence epidemiologically relevant eco-phenotypes. {T}he conventional cytogenetic method of genotyping is an impediment because it is labor intensive, requires specialized training, and can be applied only to one gender and developmental stage. {H}ere, we circumvent these limitations by developing a simple and rapid molecular method of genotyping inversion 2{R}c in {A}n. coluzzii that is both economical and field-friendly. {T}his inversion is strongly implicated in temporal and spatial adaptations to climatic and ecological variation, particularly aridity.{M}ethods{U}sing a set of tag single-nucleotide polymorphisms ({SNP}s) strongly correlated with inversion orientation, we identified those that overlapped restriction enzyme recognition sites and developed four polymerase chain reaction ({PCR}) restriction fragment length polymorphism ({RFLP}) assays that distinguish alternative allelic states at the tag {SNP}s. {W}e assessed the performance of these assays using mosquito population samples from {B}urkina {F}aso that had been cytogenetically karyotyped as well as genotyped, using two complementary high-throughput molecular methods based on tag {SNP}s. {F}urther validation was performed using mosquito population samples from additional {W}est {A}frican ({B}enin, {M}ali, {S}enegal) and {C}entral {A}frican ({C}ameroon) countries.{R}esults{O}f four assays tested, two were concordant with the 2{R}c cytogenetic karyotype > 90% of the time in all samples. {W}e recommend that these two assays be employed in tandem for reliable genotyping. {B}y accepting only those genotypic assignments where both assays agree,>99% of assignments are expected to be accurate.{C}onclusions{W}e have developed tandem {PCR}-{RFLP} assays for the accurate genotyping of inversion 2{R}c in {A}n. coluzzii. {B}ecause this approach is simple, inexpensive, and requires only basic molecular biology equipment, it is widely accessible. {T}hese provide a crucial tool for probing the molecular basis of eco-phenotypes relevant to malaria epidemiology and vector control.},
  keywords = {{A}nopheles gambiae complex ; {C}hromosomal inversion ; {I}nversion genotyping ; {M}alaria vector ; {M}olecular karyotyping ; {PCR}-{RFLP} ; {T}ag {SNP} ; {BURKINA} {FASO} ; {MALI} ; {SENEGAL} ; {BENIN} ; {CAMEROUN}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {14},
  numero = {1},
  pages = {174 [10 p.]},
  ISSN = {1756-3305},
  year = {2021},
  DOI = {10.1186/s13071-021-04657-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081166},
}