@article{fdi:010081164,
  title = {{M}olecular epidemiology of {X}anthomonas euvesicatoria strains from the {B}alkan peninsula revealed by a new multiple-locus variable-number tandem-repeat analysis scheme},
  author = {{V}ancheva, {T}. and {B}ogatzevska, {N}. and {M}oncheva, {P}. and {M}itrev, {S}. and {V}erniere, {C}. and {K}oebnik, {R}alf},
  editor = {},
  language = {{ENG}},
  abstract = {{B}acterial spot of pepper and tomato is caused by at least three species of {X}anthomonas, among them two pathovars of {X}anthomonas euvesicatoria, which are responsible for significant yield losses on all continents. {I}n order to trace back the spread of bacterial spot pathogens within and among countries, we developed the first multilocus variable number of tandem repeat analyses ({MLVA}) scheme for pepper- and tomato-pathogenic strains of {X}. euvesicatoria. {I}n this work, we assessed the repeat numbers by {DNA} sequencing of 16 tandem repeat loci and applied this new tool to analyse a representative set of 88 {X}. euvesicatoria pepper strains from {B}ulgaria and {N}orth {M}acedonia. {T}he {MLVA}-16 scheme resulted in a {H}unter-{G}aston {D}iscriminatory {I}ndex ({HGDI}) score of 0.944 and allowed to resolve 36 {MLVA} haplotypes ({MT}s), thus demonstrating its suitability for high-resolution molecular typing. {S}trains from the different regions of {B}ulgaria and {N}orth {M}acedonia were found to be widespread in genetically distant clonal complexes or singletons. {S}equence types of the variable number of tandem repeats ({VNTR}) amplicons revealed cases of size homoplasy and suggested the coexistence of different populations and different introduction events. {T}he large geographical distribution of {MT}s and the existence of epidemiologically closely related strains in different regions and countries suggest long dispersal of strains on pepper in this area.},
  keywords = {bacterial spot ; genetic diversity ; molecular typing ; pepper ; tomato ; {BULGARIE} ; {MACEDOINE}},
  booktitle = {},
  journal = {{M}icroorganisms},
  volume = {9},
  numero = {3},
  pages = {536 [19 p.]},
  year = {2021},
  DOI = {10.3390/microorganisms9030536},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081164},
}