@article{fdi:010081147,
  title = {{E}valuation of fecal immunoassays for canine {E}chinococcus infection in {C}hina},
  author = {{W}ang, {L}. {Y}. and {W}ang, {Q}. and {C}ai, {H}. {X}. and {W}ang, {H}. and {H}uang, {Y}. and {F}eng, {Y}. and {B}ai, {X}. {F}. and {Q}in, {M}. and {M}anguin, {S}ylvie and {G}avotte, {L}. and {W}u, {W}. {P}. and {F}rutos, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}uman echinococcosis is present worldwide but it is in {C}hina that disease prevalence is the highest. {I}n western {C}hina, especially in the {T}ibetan {P}lateau, the burden of echinococcosis is the most important. {D}ogs are a major definitive host of {E}chinococcus and monitoring the presence of {E}chinococcus worms in dogs is therefore essential to efficiently control the disease. {D}etection kits based on three different technologies including sandwich {ELISA}, (indirect) {ELISA}, and gold immunodiffusion, are currently marketed and used in {C}hina. {T}he objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. {F}our fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. {T}he results indicate that the performance is lower than expected, in particular in terms of sensitivity. {T}he best results were obtained with the sandwich {ELISA} technology. {T}he gold immunofiltration yielded the poorest results. {I}n all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis. {A}uthor summary {A}lthough present worldwide, human echinococcosis is at its highest prevalence in western {C}hina and particularly in the {T}ibetan {P}lateau. {C}ontrolling echinococcosis is a national priority and routine monitoring must be established. {D}ogs are the main infection source in {C}hina and surveying {E}chinococcus worms in dogs is therefore a research priority. {C}ommercial detection kits are currently in use in {C}hina to monitor the presence of {E}chinococcus in dogs. {T}he kits are based on three different technologies including sandwich {ELISA} with two monoclonal antibodies, (indirect) {ELISA}, and gold immunodiffusion. {N}ational survey programmes are essential for the control of echinococcosis and it is thus very important to assess the efficacy of these kits for the programmes. {T}his work was therefore undertaken to assess efficacy of the kits, in particular with respect to sensitivity and specificity. {F}our fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. {T}he performance was lower than expected, in particular for their sensitivity which ranged between 51.5% and 83.9% with only two samples displaying a worm burden lower than 100. {T}hree out of four kits showed non-specific cross-reactions with other parasites. {T}he best results were obtained with the sandwich {ELISA} technology, whereas gold immunofiltration yielded the poorest results. {H}owever, in all cases, further development is strongly needed to improve the performance of these kits which are key components for the control of echinococcosis.},
  keywords = {{CHINE}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {15},
  numero = {3},
  pages = {e0008690 [11]},
  ISSN = {1935-2735},
  year = {2021},
  DOI = {10.1371/journal.pntd.0008690},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081147},
}