@article{fdi:010080990, title = {{M}ulti-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscission}, author = {{F}ooyontphanich, {K}. and {M}orcillo, {F}. and {J}o{\¨e}t, {T}hierry and {D}ussert, {S}t{\'e}phane and {S}erret, {J}ulien and {C}ollin, {M}yriam and {A}mblard, {P}. and {T}angphatsornruang, {S}. and {R}oongsattham, {P}. and {J}antasuriyarat, {C}. and {V}erdeil, {J}. {L}. and {T}ranbarger, {T}imothy}, editor = {}, language = {{ENG}}, abstract = {{B}ackground {F}ruit abscission depends on cell separation that occurs within specialized cell layers that constitute an abscission zone ({AZ}). {T}o determine the mechanisms of fleshy fruit abscission of the monocot oil palm ({E}laeis guineensis {J}acq.) compared with other abscission systems, we performed multi-scale comparative transcriptome analyses on fruit targeting the developing primary {AZ} and adjacent tissues. {R}esults {C}ombining between-tissue developmental comparisons with exogenous ethylene treatments, and naturally occurring abscission in the field, {RNA}seq analysis revealed a robust core set of 168 genes with differentially regulated expression, spatially associated with the ripe fruit {AZ}, and temporally restricted to the abscission timing. {T}he expression of a set of candidate genes was validated by q{RT}-{PCR} in the fruit {AZ} of a natural oil palm variant with blocked fruit abscission, which provides evidence for their functions during abscission. {O}ur results substantiate the conservation of gene function between dicot dry fruit dehiscence and monocot fleshy fruit abscission. {T}he study also revealed major metabolic transitions occur in the {AZ} during abscission, including key senescence marker genes and transcriptional regulators, in addition to genes involved in nutrient recycling and reallocation, alternative routes for energy supply and adaptation to oxidative stress. {C}onclusions {T}he study provides the first reference transcriptome of a monocot fleshy fruit abscission zone and provides insight into the mechanisms underlying abscission by identifying key genes with functional roles and processes, including metabolic transitions, cell wall modifications, signalling, stress adaptations and transcriptional regulation, that occur during ripe fruit abscission of the monocot oil palm. {T}he transcriptome data comprises an original reference and resource useful towards understanding the evolutionary basis of this fundamental plant process.}, keywords = {{T}ranscriptome ; {F}ruit abscission ; {A}bscission zone ; {M}onocotyledon ; {M}etabolic reprogramming ; {E}laeis guineensis}, booktitle = {}, journal = {{BMC} {P}lant {B}iology}, volume = {21}, numero = {1}, pages = {92 [18 ]}, ISSN = {1471-2229}, year = {2021}, DOI = {10.1186/s12870-021-02874-1}, URL = {https://www.documentation.ird.fr/hor/fdi:010080990}, }