@article{fdi:010080947,
  title = {{V}ariation in chemical composition and antimalarial activities of two samples of {T}erminalia albida collected from separate sites in {G}uinea},
  author = {{C}amara, {A}. and {H}addad, {M}ohamed and {T}raore, {M}. {S}. and {C}hapeland-{L}eclerc, {F}. and {R}uprich-{R}obert, {G}. and {F}ouraste, {I}. and {B}alde, {M}. {A}. and {R}oyo, {J}ade and {P}arny, {M}. and {B}atigne, {P}. and {S}alon, {M}. and {C}oste, {A}. and {B}alde, {A}. {M}. and {A}ubouy, {A}gn{\`e}s},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he disparity of harvesting locations can influence the chemical composition of a plant species, which could affect its quality and bioactivity. {T}erminalia albida is widely used in traditional {G}uinean medicine whose activity against malaria has been validated in vitro and in murine models. {T}he present work investigated the antimalarial properties and chemical composition of two samples of {T}. albida collected from different locations in {G}uinea. {M}ethod {T}. albida samples were collected in different locations in {G}uinea, in {D}ubreka prefecture ({W}est maritime {G}uinea) and in {K}ankan prefecture (eastern {G}uinea). {T}he identity of the samples was confirmed by molecular analysis. {I}n vitro antiplasmodial activity of the two extracts was determined against the chloroquine resistant strain {P}f{K}1. {I}n vivo, extracts (100 mg/kg) were tested in two experimental murine models, respectively infected with {P}. chabaudi chabaudi and {P}. berghei {ANKA}. {T}he chemical composition of the two samples was assessed by ultra-high-performance liquid chromatography coupled to high resolution mass spectrometry. {R}esults {I}n vitro, the {D}ubreka sample ({T}a{D}) was more active with an {IC}50 of 1.5 mu g/m{L} versus 8.5 mu g/m{L} for the extract from {K}ankan ({T}a{K}). {I}n vivo, the antiparasitic effect of {T}a{D} was substantial with 56% of parasite inhibition at {D}ay 10 post-infection in {P}. chabaudi infection and 61% at {D}ay 8 in {P}. berghei model, compared to 14 and 19% inhibition respectively for the treatment with {T}a{K}. {I}n addition, treatment with {T}a{D} further improved the survival of {P}. berghei infected-mice by 50% at {D}ay 20, while the mortality rate of mice treated with {T}ak was similar to the untreated group. {T}he {LC}/{MS} analysis of the two extracts identified 38 compounds, 15 of which were common to both samples while 9 and 14 other compounds were unique to {T}a{D} and {T}a{K} respectively. {C}onclusion {T}his study highlights the variability in the chemical composition of the species {T}. albida when collected in different geographical locations. {T}hese chemical disparities were associated with variable antimalarial effects. {F}rom a public health perspective, these results underline the importance of defining chemical fingerprints related to botanical species identification and to biological activity, for the plants most commonly used in traditional medicine.},
  keywords = {{T}erminalia albida ; {M}alaria ; {G}eographical location ; {UHPLC}-{HRMS} ; {P}lant molecular analysis ; {P}lasmodium berghei ; {P}lasmodium chabaudi ; {GUINEE}},
  booktitle = {},
  journal = {{BMC} {C}omplementary {M}edicine and {T}herapies},
  volume = {21},
  numero = {1},
  pages = {64 [12p.]},
  year = {2021},
  DOI = {10.1186/s12906-021-03231-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080947},
}