@article{fdi:010080933,
  title = {{N}ovel heavy metal resistance gene clusters are present in the genome of {C}upriavidus neocaledonicus {STM} 6070, a new species of {M}imosa pudica microsymbiont isolated from heavy-metal-rich mining site soil},
  author = {{K}lonowska, {A}gnieszka and {M}oulin, {L}ionel and {A}rdley, {J}. {K}. and {B}raun, {F}. and {G}ollagher, {M}. {M}. and {Z}andberg, {J}. {D}. and {M}arinova, {D}. {V}. and {H}untemann, {M}. and {R}eddy, {T}. {B}. {K}. and {V}arghese, {N}. {J}. and {W}oyke, {T}. and {I}vanova, {N}. and {S}eshadri, {R}. and {K}yrpides, {N}. and {R}eeve, {W}. {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}upriavidus strain {STM} 6070 was isolated from nickel-rich soil collected near {K}oniambo massif, {N}ew {C}aledonia, using the invasive legume trap host {M}imosa pudica. {STM} 6070 is a heavy metal-tolerant strain that is highly effective at fixing nitrogen with {M}. pudica. {H}ere we have provided an updated taxonomy for {STM} 6070 and described salient features of the annotated genome, focusing on heavy metal resistance ({HMR}) loci and heavy metal efflux ({HME}) systems. {R}esults: {T}he 6,771,773 bp high-quality-draft genome consists of 107 scaffolds containing 6118 protein-coding genes. {ANI} values show that {STM} 6070 is a new species of {C}upriavidus. {T}he {STM} 6070 symbiotic region was syntenic with that of the {M}. pudica-nodulating {C}upriavidus taiwanensis {LMG} 19424{T}. {I}n contrast to the nickel and zinc sensitivity of {C}. taiwanensis strains, {STM} 6070 grew at high {N}i2+ and {Z}n2+ concentrations. {T}he {STM} 6070 genome contains 55 genes, located in 12 clusters, that encode {HMR} structural proteins belonging to the {RND}, {MFS}, {CHR}, {ARC}3, {CDF} and {P}-{ATP}ase protein superfamilies. {T}hese {HMR} molecular determinants are putatively involved in arsenic (ars), chromium (chr), cobalt-zinc-cadmium (czc), copper (cop, cup), nickel (nie and nre), and silver and/or copper (sil) resistance. {S}even of these {HMR} clusters were common to symbiotic and non-symbiotic {C}upriavidus species, while four clusters were specific to {STM} 6070, with three of these being associated with insertion sequences. {W}ithin the specific {STM} 6070 {HMR} clusters, three novel {HME}-{RND} systems (nie{IC} cep nie{BA}, czc{C}2{B}2{A}2, and hmx{B} zne{AC} zne{R} hmx{S}) were identified, which constitute new candidate genes for nickel and zinc resistance. {C}onclusions: {STM} 6070 belongs to a new {C}upriavidus species, for which we have proposed the name {C}upriavidus neocaledonicus sp. nov.. {STM}6070 harbours a p{S}ym with a high degree of gene conservation to the p{S}yms of {M}. pudica-nodulating {C}. taiwanensis strains, probably as a result of recent horizontal transfer. {T}he presence of specific {HMR} clusters, associated with transposase genes, suggests that the selection pressure of the {N}ew {C}aledonian ultramafic soils has driven the specific adaptation of {STM} 6070 to heavy-metal-rich soils via horizontal gene transfer.},
  keywords = {{R}hizobia ; {C}upriavidus ; {N}ickel tolerance ; {HGT} ; {M}imosa ; {R}hizobial ; biogeography ; {H}eavy metal resistance ; {H}eavy metal efflux ; {NOUVELLE} {CALEDONIE} ; {KONIAMBO} {MASSIF}},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {21},
  numero = {1},
  pages = {214 [18 p.]},
  ISSN = {1471-2164},
  year = {2020},
  DOI = {10.1186/s12864-020-6623-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080933},
}