@article{fdi:010080891,
  title = {{A}pplication of the {FTA} elute card coupled with visual colorimetric loop-mediated isothermal amplification for the rapid diagnosis of {S}treptococcus agalactiae in farmed tilapia ({O}reochromis niloticus)},
  author = {{P}epey, {E}. and {T}aukhid, {T}. and {K}eck, {N}. and {L}usiastuti, {A}. and {A}varre, {J}ean-{C}hristophe and {S}undari, {G}. and {S}arter, {S}. and {C}aruso, {D}omenico},
  editor = {},
  language = {{ENG}},
  abstract = {{A} method combining the {FTA} {E}lute card and visual colorimetric loop-mediated isothermal amplification ({FTA}-e/{LAMP}) was tested to diagnose {S}treptococcus agalactiae infections in vitro and in vivo. {FTA}-e/{LAMP} consists of two main steps: first, the {FTA} card is used to extract {DNA} and then a colorimetric loop-mediated isothermal amplification ({LAMP}) reaction is carried out on the extracted {DNA}. {I}n vitro sensitivity was 1.9 x 10(2) {CFU}/m{L}, and regarding specificity, all nine {S}. agalactiae strains tested positive. {A}ll {S}treptococcus spp. tested negative, except for {S}. dysgalactiae, thereby indicating the need for another set of primers to distinguish this species from {S}. agalactiae. {T}o diagnose {S}. agalactiae infections using {FTA}-e/{LAMP} in vivo, two experimental trials on juvenile {O}reochromis niloticus infected with bovine or piscine strains were carried out. {S}ensitivity in symptomatic fish was 100%, and 50.7% of fish without signs were positive. {A}ll negative control fish tested negative (n = 28). {N}o bacteria were detected after 16 days post-infection (dpi). {A}ccuracy during the first week (1-7 dpi) was 89% and decreased to 44% thereafter (10-22 dpi). {FTA}-e/{LAMP} results suggest that this method is a promising tool for early and fast diagnosis of {S}. agalactiae on tilapia farms.},
  keywords = {bacterial fish disease ; field diagnosis ; {LAMP} ; sensitivity ; specificity ; {INDONESIE}},
  booktitle = {},
  journal = {{J}ournal of {F}ish {D}iseases},
  volume = {44},
  numero = {5},
  pages = {505--512},
  ISSN = {0140-7775},
  year = {2021},
  DOI = {10.1111/jfd.13337},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080891},
}