@article{fdi:010080795,
  title = {{M}olecular identification of diminazene aceturate resistant trypanosomes in tsetse flies from {Y}oko in the {C}entre region of {C}ameroon and its epidemiological implications},
  author = {{S}imo, {G}. and {K}emta {M}agang, {E}.{M}. and {M}ewamba, {E}.{M}. and {F}arikou, {O}. and {K}amga, {R}.{M}.{N}. and {T}ume, {C}. and {S}olano, {P}hilippe and {R}avel, {S}ophie},
  editor = {},
  language = {{ENG}},
  abstract = {{A}frican animal trypanosomiases are caused by trypanosomes cyclically or mechanically transmitted by tsetse and other biting flies. {A}lthough molecular tools have been developed to identify drug-resistant trypanosomes in mammals, little or no investigation on drug-resistance has been undertaken on trypanosomes harbored by tsetse flies. {M}oreover, no data on mechanical vectors of {A}frican trypanosomes is available in most endemic areas of {C}ameroon. {T}his study was designed to update our knowledge on the cyclical and mechanical vectors of {A}frican trypanosomes, and using molecular tools to identify different trypanosome species as well as diminazene aceturate resistant trypanosomes in tsetse flies trapped at {Y}oko in the {C}entre region of {C}ameroon. {F}or this study, traps were used to catch tsetse and mechanical vectors of {A}frican trypanosomes. {T}he flies trapped were counted and identified by sex and species. {DNA} was extracted from tsetse and species-specific primers were used to identify different trypanosome species. {PCR}-{RFLP} was used to detect diminazene aceturate resistant strains of {T}rypanosoma congolense. {I}n all, 454 flies comprising 168 (37%) {T}abanus spp., 71 (15.6%) {S}tomoxys spp. and 215 (47.4%) tsetse fly (i.e. 107 (49.8%) {G}lossina fusca congolensis, 71 (33%) {G}lossina fusca fusca and 37 (17.2%) {G}lossina palpalis palpalis) were trapped. {T}rypanosome infections were identified in 12.6% (27/215) of tsetse flies: 13 in {G}. f. congolensis, 6 in {G}. p. palpalis and 5 in {G}. f. fusca. {F}rom 24 {T}. congolense positive samples, {PCR}-{RFLP} was successful on 37.5% of the samples. {F}our samples (16.2%) harbored {T}. congolense strains that were resistant to diminazene aceturate while the remaining samples had drug-sensitive strains. {T}hese results show for the first time the applicability of molecular tools for the identification of drug-resistant trypanosomes in tsetse. {T}hey revealed the existence of diminazene aceturate resistant strains of {T}. congolense in the tsetse-infested area of {Y}oko in the {C}entre region of {C}ameroon. {D}etection of drug-resistant trypanosomes in tsetse may enable scientists to map with accuracy specific areas where these parasites are transmitted. {W}ith such mapping, control strategies against {A}frican trypanosomiases could be improved by adapting control measures according to drug resistance distribution},
  keywords = {{CAMEROUN} ; {YOKO}},
  booktitle = {},
  journal = {{P}arasite {E}pidemiology and {C}ontrol},
  volume = {9},
  numero = {},
  pages = {e00135 [9 ]},
  ISSN = {2405-6731},
  year = {2020},
  DOI = {10.1016/j.parepi.2020.e00135},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080795},
}