Diop M.M. auteur aut IRD Chandre Fabrice auteur aut IRD Rossignol Marie auteur aut IRD Porciani Angélique auteur aut IRD Chateau M. auteur aut IRD Moiroux Nicolas auteur aut IRD Pennetier Cédric auteur aut IRD text report Litérature grise eng text/pdf reformatted digital access The massive use of insecticide-treated nets (ITNs) has drastically changed the environment for malaria vector mosquitoes, challenging their host-seeking behaviour and biting success. Here, we investigated the effect of a brief exposure to an ITN on the biting behaviour of Anopheles mambiae mosquitoes and the interaction between such behaviour and the kdr mutation that confers resistance to pyrethroids. To this aim, we developed a video assay to study the biting behaviour of mosquitoes with similar genetic background, but different kdr locus genotypes (SS i.e. homozygous susceptible, RS i.e. heterozygous and RR i.e. homozygous resistant), after a brief exposure to either control untreated nets or one of two types of pyrethroid-treated nets (deltamethrin or permethrin). In presence of untreated nets, the kdr mutation did not influence mosquito blood feeding success but caused differences in feeding and prediuresis durations and blood meal size. Exposure to deltamethrin ITN decreased the blood feeding success rate of RR and RS mosquitoes, whereas in presence of permethrin ITN, the kdr mutation increased the blood-feeding success of mosquitoes. Exposure to the two types of pyrethroid-treated nets reduced feeding duration, prediuresis duration and blood meal size of all three genotypes. Our study demonstrates a complex interaction between insecticide exposure and the kdr mutation on the biting behavior of mosquitoes, which may substantially impact malaria vector fitness and disease transmission. specialized KENYA BURKINA FASO FRANCE 052LUTIN01 2020 653980 en ligne [20 ] en ligne [20 ] en ligne [20 ] https://www.documentation.ird.fr/hor/fdi:010080718 10.1101/653980 [F B010080718] https://www.documentation.ird.fr/hor/fdi:010080718 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers21-01/010080718.pdf IRD - Base Horizon / Pleins textes 2020-12-22 2025-03-20 fdi:010080718 fre