@article{fdi:010080631,
  title = {{P}lasmodium falciparum gametocyte carriage in symptomatic patients shows significant association with genetically diverse infections, anaemia, and asexual stage density},
  author = {{S}ondo, {P}. and {B}ihoun, {B}. and {T}ahita, {M}. {C}. and {D}erra, {K}. and {R}ouamba, {T}. and {D}iallo, {S}. {N}. and {K}azienga, {A}. and {I}lboudo, {H}. and {V}alea, {I}. and {T}arnagda, {Z}. and {S}orgho, {H}. and {L}ef{\`e}vre, {T}hierry and {T}into, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{M}ulti-genotype malaria infections are frequent in endemic area, and people commonly harbour several genetically distinct {P}lasmodium falciparum variants. {T}he influence of genetic multiplicity and whether some specific genetic variants are more or less likely to invest into gametocyte production is not clearly understood. {T}his study explored host and parasite-related risk factors for gametocyte carriage, and the extent to which some specific {P}. falciparum genetic variants are associated with gametocyte carriage.{M}ethods{G}ametocytes and asexual forms were detected by light microscopy on thick smears collected between 2010 and 2012 in {N}anoro, {B}urkina {F}aso. {M}erozoite surface protein 1 and 2 were genotyped by nested {PCR} on clinical samples. {A}ssociations between gametocyte carriage and factors, including multiplicity of infection, parasite density, patient age, gender, haemoglobin ({H}b) level, and body temperature were assessed. {T}he relationship between the presence of a particular msp1 and msp2 genetic variants and gametocyte carriage was also explored.{R}esults{O}f the 724 samples positive to {P}. falciparum and successfully genotyped, gametocytes were found in 48 samples (6.63%). {T}here was no effect of patient gender, age and body temperature on gametocyte carriage. {H}owever, the probability of gametocyte carriage significantly increased with increasing values of multiplicity of infection ({MOI}). {F}urthermore, there was a negative association between parasite density and gametocyte carriage. {MOI} decreased with parasite density in gametocyte-negative patients, but increased in gametocyte carriers. {T}he probability of gametocyte carriage decreased with {H}b level. {F}inally, the genetic composition of the infection influenced gametocyte carriage. {I}n particular, the presence of {RO}33 increased the odds of developing gametocytes by 2 while the other allelic families {K}1, {MAD}20, {FC}27, and 3{D}7 had no significant impact on the occurrence of gametocytes in infected patients.{C}onclusion{T}his study provides insight into potential factors influencing gametocyte production in symptomatic patients. {T}he findings contribute to enhance understanding of risk factors associated with gametocyte carriage in humans.{T}rial registration {NCT}01232530.},
  keywords = {{M}alaria ; {P}lasmodium falciparum ; {G}ametocyte ; msp1 ; msp2 ; {M}ultiplicity of infection ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {20},
  numero = {1},
  pages = {31 [11 ]},
  year = {2021},
  DOI = {10.1186/s12936-020-03559-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080631},
}