Tan E. Y. W. auteur aut IRD Quek Z. B. R. auteur aut IRD Neo M. L. auteur aut IRD Fauvelot Cécile auteur aut IRD Huang D. W. auteur aut IRD text journalArticle eng text/pdf born digital access Giant clams are conspicuous bivalves inhabiting Indo-Pacific reefs. Since Rosewater's seminal paper in 1965, the number of giant clam species described or resurrected has exactly doubled. The increased number of species reported and accessibility to genetic material of rare or uncommon species therefore call for a reappraisal of the phylogenetic relationships within the Tridacninae subfamily. Here, we aim to reconstruct the evolutionary relationships among all 12 extant species by performing a comprehensive phylogenetic analysis of mitochondrial genome and nuclear 18S rRNA data from a combination of genome skimming, Sanger sequencing and previously published sequences. Comparing the mitogenomes among Tridacninae species, we report two new findings: (1) the T. crocea mitogenome length obtained here (18,266 bp) is shorter than previously known, and (2) the mitochondrial gene order in T. crocea and T. squamosa differs from the other species. Our phylogeny based on a concatenated 16-gene dataset (15 mitochondrial markers and nuclear 18S rRNA) reveals highly supported relationships within and between the three subgenera, Tridacna (Tridacna), Tridacna (Persikima) and Tridacna (Chametrachea). Overall, the inclusion of new molecular markers greatly improves the confidence and support for the subfamily's phylogeny. The availability of this comprehensive phylogenetic dataset serves as the foremost baseline of Tridacninae relationships to support future studies examining giant clam systematics, ecology and conservation. specialized Coral reef Hippopus Indo-Pacific Next-generation sequencing Phylogenetics Systematics Tridacna PACIFIQUE OCEAN INDIEN 034 020 41 3 497-510 2021 0722-4028 https://www.documentation.ird.fr/hor/fdi:010080593 10.1007/s00338-020-02039-w 0722-4028 [F B010080593] https://www.documentation.ird.fr/hor/fdi:010080593 https://www.documentation.ird.fr/intranet/publi/2021/02/010080593.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2021-03-10 2025-02-24 fdi:010080593 fre