@article{fdi:010080508,
  title = {{L}aboratory development of a simple stool sample processing method diagnosis of pediatric tuberculosis using {X}pert {U}ltra},
  author = {{L}ounnas, {M}anon and {D}iack, {A}. and {N}icol, {M}. {P}. and {E}yangoh, {S}. and {W}obudeya, {E}. and {M}arcy, {O}. and {G}odreuil, {S}. and {B}onnet, {M}aryline},
  editor = {},
  language = {{ENG}},
  abstract = {{S}tool samples are alternatives to respiratory samples for bacteriological confirmation of childhood tuberculosis but require intensive laboratory processing before molecular testing to remove {PCR} inhibitors and debris. {W}e aimed to develop a centrifuge-free processing method for use in resource-limited settings based on a sucrose-flotation method that showed good sensitivity for childhood tuberculosis diagnosis. {I}n an in vitro study using {X}pert {MTB}/{RIF} {U}ltra on stool samples spiked with defined bacterial concentrations of {M}ycobacterium tuberculosis ({MTB}), we compared different simplification parameters to the reference sucroseflotation method. {B}est methods were selected based on the rate of invalid/error results and on sensitivity, compared to the reference method on stools spiked at 10(3) colony forming units ({CFU})/g {MTB}. {F}or final selection, we tested the best parameter combinations at 10(2) {CFU}/g. {O}ut of 13 different parameter combinations, three were tested at 10(2) {CFU}/g. {T}he best combination used 0.5 g stool, manual shaking, no filtration, 30-min sedimentation, and a 1:3.6 dilution ratio. {T}his method gave 10% invalid/error results and a sensitivity of 70% vs 63% at 10(3) {CFU}/g and 53% vs 58% at 10(2) {CFU}/g compared to the reference method. {T}his pre-clinical study was able to develop a centrifuge-free processing method to facilitate stool {X}pert {U}ltra testing.},
  keywords = {{D}iagnosis ; {X}pert {MTB}/{R}if ultra ; {C}hildhood tuberculosis ; {S}tool},
  booktitle = {},
  journal = {{T}uberculosis},
  volume = {125},
  numero = {},
  pages = {102002 [9 p.]},
  ISSN = {1472-9792},
  year = {2020},
  DOI = {10.1016/j.tube.2020.102002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080508},
}