da Silva J. A. T. auteur aut IRD Wicaksono A. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access Main conclusion This paper reviews the cryopreservation of the ornamental, carnation (Dianthus caryophyllus L.), as an important method for the long-term preservation of this plant's germplasm. Carnation (Dianthus caryophyllus L.) is an important ornamental plant that is used as a potted plant as well as a cut flower. Important Dianthus germplasm would benefit from long-term strategies such as cryopreservation. Unlike the in vitro tissue culture literature of this ornamental, which has been studied in considerable detail, and with several genetic transformation protocols, surprisingly, the literature on its cryopreservation is still fairly scant, with barely two dozen or so studies, mostly having employed shoot tips. Early (< 2007) and more recent (2007-2020) cryopreservation techniques for carnation, including ultra-rapid cooling, encapsulation-vitrification, and encapsulation-dehydration, efficiently replaced programmed slow cooling processes used in early studies in the 1980s. Two large gaps (1997-2006, and 2016-2020) in which no carnation cryopreservation studies were published, requires future studies to cover new knowledge to fill gaps in information. Carnation cryopreservation research would benefit from testing a wide range of in vitro explants, new techniques such as the cryo-mesh, improved regeneration protocols for post-cryopreserved material, and the use of low-temperature storage as a mid- to long-term complementary germplasm storage strategy. This mini-review provides details of what has been achieved thus far and future objectives that could fortify cryopreservation research of this ornamental, as well as provide a robust long-term germplasm repository. specialized Cryoprotectant Loading solution Organogenesis PVS2 Somatic and zygotic embryos Plant tissue culture Vitrification 076 084 252 6 105 [9 p.] 2020 0032-0935 https://www.documentation.ird.fr/hor/fdi:010080022 0032-0935 [F B010080022] https://www.documentation.ird.fr/hor/fdi:010080022 https://www.documentation.ird.fr/intranet/publi/2020/12/010080022.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2021-01-14 2021-01-14 fdi:010080022 fre