@article{fdi:010079942,
  title = {{H}igh-throughput genotyping of common chromosomal inversions in the afrotropical malaria mosquito {A}nopheles funestus},
  author = {{L}ukindu, {M}. and {L}ove, {R}. {R}. and {G}uelbeogo, {M}. {W}. and {S}mall, {S}. {T}. and {S}tephens, {M}. {T}. and {C}ampbell, {N}. {R}. and {S}agnon, {N}. and {C}ostantini, {C}arlo and {B}esansky, {N}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}imple {S}ummary {C}hromosomal inversions occur when a segment of {DNA} breaks in two places, rotates 180 degrees, and reattaches. {I}nversions can protect sets of genetic variants, including those adapted to local conditions, from being split up in the random shuffling that occurs when genetic information is transmitted from one generation to the next. {F}or this reason, inversions can play a role in local adaptation and range expansion. {L}ike many malaria mosquitoes, {A}nopheles funestus, which plays a major role in transmitting malaria in sub-{S}aharan {A}frica, carries several common chromosomal inversions. {T}hree of these inversions have been implicated in traits such as indoor resting behavior, which affects their rate of contact with both humans and insecticide-based interventions. {I}nversions therefore have relevance for malaria epidemiology and control. {I}nversions are traditionally identified by examining chromosomes under a microscope, but this method is difficult and time-consuming, and only applicable to a subset of female adult mosquitoes. {T}o overcome this limitation, we developed high-throughput {DNA}-based diagnostic methods to predict the presence of inversions. {T}he availability of these methods will allow scientists to more easily identify inversions in larger samples of mosquitoes, from all life stages and both sexes, which will help us determine how inversions are affecting malaria transmission. {P}olymorphic chromosomal inversions have been implicated in local adaptation. {I}n anopheline mosquitoes, inversions also contribute to epidemiologically relevant phenotypes such as resting behavior. {P}rogress in understanding these phenotypes and their mechanistic basis has been hindered because the only available method for inversion genotyping relies on traditional cytogenetic karyotyping, a rate-limiting and technically difficult approach that is possible only for the fraction of the adult female population at the correct gonotrophic stage. {H}ere, we focus on an understudied malaria vector of major importance in sub-{S}aharan {A}frica, {A}nopheles funestus. {W}e ascertain and validate tag single nucleotide polymorphisms ({SNP}s) using high throughput molecular assays that allow rapid inversion genotyping of the three most common {A}n. funestus inversions at scale, overcoming the cytogenetic karyotyping barrier. {T}hese same inversions are the only available markers for distinguishing two {A}n. funestus ecotypes that differ in indoor resting behavior, {F}olonzo and {K}iribina. {O}ur new inversion genotyping tools will facilitate studies of ecotypic differentiation in {A}n. funestus and provide a means to improve our understanding of the roles of {F}olonzo and {K}iribina in malaria transmission.},
  keywords = {{A}nopheles funestus ; chromosomal inversion polymorphism ; polytene ; chromosome analysis ; inversion genotyping ; karyotyping ; malaria vector ; tag {SNP} ; {BURKINA} {FASO} ; {GHANA} ; {KENYA} ; {TANZANIA} ; {OUGANDA} ; {MOZAMBIQUE} ; {ZAMBIE} ; {ZONE} {TROPICALE} ; {ZONE} {SOUDANNIENNE} ; {ZONE} {GUINEENNE}},
  booktitle = {},
  journal = {{I}nsects},
  volume = {11},
  numero = {10},
  pages = {693 [16 p.]},
  year = {2020},
  DOI = {10.3390/insects11100693},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079942},
}