@article{fdi:010079832,
  title = {{E}valuation of the {G}eno{T}ype {NTM}-{DR} assay performance for the identification and molecular detection of antibiotic resistance in {M}ycobacterium abscessus complex},
  author = {{B}ouzinbi, {N}. and {M}arcy, {O}. and {B}ertolotti, {T}. and {C}hiron, {R}. and {B}emer, {P}. and {P}estel-{C}aron, {M}. and {P}euchant, {O}. and {G}uet-{R}evillet, {H}. and {F}angous, {M}. {S}. and {H}ery-{A}rnaud, {G}. and {O}uedraogo, {A}. {S}. and {B}anuls, {A}nne-{L}aure and {G}odreuil, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he first objective of this study was to determine the {G}eno{T}ype {NTM}-{DR} assay performance for subspecies identification in {M}ycobacterium abscessus complex isolates. {T}he second objective was to evaluate the {G}eno{T}ype {NTM}-{DR} assay ability to detect clarithromycin and amikacin resistance in {M}.abscessus complex isolates compared with drug susceptibility testing ({DST}) and {PCR} sequencing of the erm(41),rrl and rrs genes. {T}he concordance between the {G}eno{T}ype {NTM}-{DR} and {MLST} results concerning subspecies identification was 100%. {T}he wild type and mutated alleles of the rrlandrrs genes were detected by the {G}eno{T}ype {NTM}-{DR} assay and {PCR} sequencing with 100% (115/115) agreement. {S}imilarly, 100% concordance between {G}eno{T}ype {NTM}-{DR} and {DST} was observed for clarithromycin and amikacin testing. {S}ensitivity for the detection of clarithromycin and amikacin resistance was 100%. {T}he {G}eno{T}ype {NTM}-{DR} assay provides a robust and complementary tool to the gold standard methods ({MLST} and broth microdilution) for subspecies identification and drug resistance detection.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {15},
  numero = {9},
  pages = {e0239146 [5 p.]},
  ISSN = {1932-6203},
  year = {2020},
  DOI = {10.1371/journal.pone.0239146},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079832},
}