@article{fdi:010079809,
  title = {{P}hylogenetic distribution and evolutionary dynamics of nod and {T}3{SS} genes in the genus {B}radyrhizobium},
  author = {{T}eulet, {A}lbin and {G}ully, {D}jamel and {R}ouy, {Z}. and {C}amuel, {A}. and {K}oebnik, {R}alf and {G}iraud, {E}ric and {L}assalle, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}radyrhizobium are abundant soil bacteria and the major symbiont of legumes. {T}he recent availability of {B}radyrhizobium genome sequences provides a large source of information for analysis of symbiotic traits. {I}n this study, we investigated the evolutionary dynamics of the nodulation genes (nod) and their relationship with the genes encoding type {III} secretion systems ({T}3{SS}) and their effectors among bradyrhizobia. {B}ased on the comparative analysis of 146 {B}radyrhizobium genome sequences, we identified six different types of {T}3{SS} gene clusters. {T}he two predominant cluster types are designated {R}hc{I}a and {R}hc{I}b and both belong to the {R}hc{I}-{T}3{SS} family previously described in other rhizobia. {T}hey are found in 92/146 strains, most of them also containing nod genes. {R}hc{I}a and {R}hc{I}b gene clusters differ in the genes they carry: while the translocon-encoding gene nop{X} is systematically found in strains containing {R}hc{I}b, the nop{E} and nop{H} genes are specifically conserved in strains containing {R}hc{I}a, suggesting that these last two genes might functionally substitute nop{X} and play a role related to effector translocation. {P}hylogenetic analysis suggests that bradyrhizobia simultaneously gained nod and {R}hc{I}-{T}3{SS} gene clusters via horizontal transfer or subsequent vertical inheritance of a symbiotic island containing both. {S}equence similarity searches for known {N}op effector proteins in bradyrhizobial proteomes revealed the absence of a so-called core effectome, i.e. that no effector is conserved among all {B}radyrhizobium strains. {H}owever, {N}op{M} and {SUMO} proteases were found to be the main effector families, being represented in the majority of the genus. {T}his study indicates that bradyrhizobial {T}3{SS}s might play a more significant symbiotic role than previously thought and provides new candidates among {T}3{SS} structural proteins and effectors for future functional investigations.},
  keywords = {symbiosis ; legume ; rhizobium ; effector ; nodulation ; type {III} secretion system},
  booktitle = {},
  journal = {{M}icrobial {G}enomics},
  volume = {6},
  numero = {9},
  pages = {000407 [18 p.]},
  ISSN = {2057-5858},
  year = {2020},
  DOI = {10.1099/mgen.0.000407},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079809},
}