@article{fdi:010079739,
  title = {{I}nversion genotyping in the {A}nopheles gambiae complex using high-throughput array and sequencing platforms},
  author = {{L}ove, {R}. {R}. and {P}ombi, {M}. and {G}uelbeogo, {M}. {W}. and {C}ampbell, {N}. {R}. and {S}tephens, {M}. {T}. and {D}abire, {R}. {K}. and {C}ostantini, {C}arlo and della {T}orre, {A}. and {B}esansky, {N}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}hromosomal inversion polymorphisms have special importance in the{A}nopheles gambiaecomplex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. {T}he study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. {T}o overcome this barrier, we ascertained tag single nucleotide polymorphisms ({SNP}s) that are highly correlated with inversion status (inverted or standard orientation). {W}e compared the performance of the tag {SNP}s using two alternative high throughput molecular genotyping approachesvs.traditional cytogenetic karyotyping of the same 960 individual{A}n. gambiaeand{A}n. coluzziimosquitoes sampled from {B}urkina {F}aso, {W}est {A}frica. {W}e show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. {G}iven the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag {SNP}s has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation of{A}n. gambiaeand{A}n. coluzziito environmental heterogeneities.},
  keywords = {{A}nopheles gambiae ; chromosomal inversion polymorphism ; polytene ; chromosome analysis ; inversion genotyping ; karyotyping ; malaria vector ; tag {SNP} ; {BURKINA} {FASO} ; {ZONE} {SOUDANNIENNE}},
  booktitle = {},
  journal = {{G}3 : {G}enes {G}enomes {G}enetics},
  volume = {10},
  numero = {9},
  pages = {3299--3307},
  ISSN = {2160-1836},
  year = {2020},
  DOI = {10.1534/g3.120.401418},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079739},
}