@article{fdi:010079709, title = {{N}ew microsatellite {DNA} markers to resolve population structure of the convict surgeonfish, {A}canthurus triostegus, and cross-species amplifications on thirteen other {A}canthuridae}, author = {{G}rulois, {D}. and {H}ogan, {R}. {I}. and {P}aygambar, {S}. and {P}lanes, {S}. and {F}auvelot, {C}{\'e}cile}, editor = {}, language = {{ENG}}, abstract = {{M}icrosatellites are widely used to investigate connectivity and parentage in marine organisms. {D}espite surgeonfish ({A}canthuridae) being dominant members of most reef fish assemblages and having an ecological key role in coral reef ecosystems, there is limited information describing the scale at which populations are connected and very few microsatellite markers have been screened. {H}ere, we developed fourteen microsatellite markers for the convict surgeonfish{A}canthurus triosteguswith the aim to infer its genetic connectivity throughout its distribution range. {G}enetic diversity and variability was tested over 152 fishes sampled from four locations across the {I}ndo-{P}acific: {M}ayotte ({W}estern {I}ndian {O}cean), {P}apua {N}ew {G}uinea and {N}ew {C}aledonia ({S}outhwestern {P}acific {O}cean), and {M}oorea ({F}rench {P}olynesia). {O}ver all locations, the number of alleles per locus varied from 5 to 24 per locus, and expected heterozygosities ranged from 0.468 to 0.941. {S}ignificant deviations from {H}ardy-{W}einberg equilibrium were detected for two loci in two to three locations and were attributed to the presence of null alleles. {T}hese markers revealed for the first time a strong and significant distinctiveness between {I}ndian {O}cean and {P}acific {O}cean{A}. triosteguspopulations. {W}e further conducted cross-species amplification tests in 13 {P}acific congener species to investigate the possible use of these microsatellites in other {A}canthuridae species. {T}he phylogenetic placement of{A}. triostegusbranching off from the clade containing nearly all{A}canthurus + {C}tenochaetusspecies likely explain the rather good transferability of these microsatellite markers towards other {A}canthuridae species. {T}his suggests that this fourteen new microsatellite loci will be helpful tools not only for inferring population structure of various surgeonfish but also to clarify systematic relationships among {A}canthuridae.}, keywords = {{C}oral reef fish ; {M}icrosatellites ; {C}onnectivity ; {I}ndo-pacific ; {G}enetic structure ; {S}urgeonfish ; {OCEAN} {INDIEN} ; {PACIFIQUE} ; {MAYOTTE} ; {PAPOUASIE} {NOUVELLE} {GUINEE} ; {NOUVELLE} {CALEDONIE} ; {POLYNESIE} {FRANCAISE} ; {MOOREA}}, booktitle = {}, journal = {{M}olecular {B}iology {R}eports}, volume = {47}, numero = {10}, pages = {8243--8250}, ISSN = {0301-4851}, year = {2020}, DOI = {10.1007/s11033-020-05773-0}, URL = {https://www.documentation.ird.fr/hor/fdi:010079709}, }