Deep modifications of the microbiome of rice roots infected by the parasitic nematode Meloidogyne graminicola in highly infested fields in Vietnam

Deep modifications of the microbiome of rice roots infected by the parasitic nematode Meloidogyne graminicola in highly infested fields in Vietnam Masson A. S. auteur aut IRD Bich H. H. auteur aut IRD Simonin Marie auteur aut IRD Thi H. N. auteur aut IRD Czernic P. auteur aut IRD Moulin Lionel auteur aut IRD Bellafiore Stéphane auteur aut IRD text journalArticle eng text/pdf born digital access Meloidogyne graminicola, also known as the rice root-knot nematode, is one of the most damaging plant-parasitic nematode, especially on rice. This obligate soilbome parasite induces the formation of galls that disturb the root morphology and physiology. Its impact on the root microbiome is still not well described. Here, we conducted a survey in Northern Vietnam where we collected infected (with galls) and non-infected root tips from the same plants in three naturally infested fields. Using a metabarcoding approach, we discovered that M. graminicola infection caused modifications of the root bacterial community composition and network structure. Interestingly, we observed in infected roots a higher diversity and species richness (+24% observed ESVs) as well as a denser and more complex co-occurrence network (+44% nodes and +136% links). We identified enriched taxa that include several hubs, which could serve as potential indicators or biocontrol agents of the nematode infection. Moreover, the community of infected roots is more specific suggesting changes in the functional capabilities to survive in the gall environment. We thus describe the signature of the gall microbiome (the 'gallobiome, with shifting abundances and enrichments that lead to a strong restructuration of the root microbiome. specialized lowland rice root-knot nematode gall microbiome bacterial community co-occurrence network metabarcoding VIET NAM 076 074 FEMS Microbiology Ecology 96 7 fiaa099 [14 p.] 2020 0168-6496 https://www.documentation.ird.fr/hor/fdi:010079587 10.1093/femsec/fiaa099 0168-6496 [F B010079587] https://www.documentation.ird.fr/hor/fdi:010079587 https://www.documentation.ird.fr/intranet/publi/2020/09/010079587.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-10-09 2025-02-24 fdi:010079587 fre